Human UACA ELISA Kit

Alternative name
Human KIAA1561 ELISA Kit;Human NUCLING ELISA Kit;Human uveal autoantigen with coiled-coil domains and ankyrin repeats ELISA Kit;Human nuclear membrane binding protein ELISA Kit;
Catalog
E002718
species
Human
GeneUACA
Standard Curve
Other Species Mouse Uaca ELISA Kit Bovine UACA ELISA Kit Canine UACA ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of anti-UACA. No significant cross-reactivity or interference between anti-UACA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between anti-UACA and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman UACA ELISA Kit allows for the in vitro quantitative determination of UACA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: Anti-UACA ELISA kit applies the competitive enzyme immunoassay technique utilizing a UACA antigen and an anti-UACA-HRP conjugate. The assay sample and buffer are incubated together with anti-UACA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-UACA concentration since anti-UACA from samples and anti-UACA-HRP conjugate compete for the UACA antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-UACA from the sample, fewer sites are left to bind anti-UACA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-UACA concentration in each sample is interpolated from this standard curve.
Human Uveal autoantigen with coiled-coil domains and ankyrin repeats Protein information
Uniprot ID UACA_HUMAN
Uniprot AC Q9BZF9; G3XAG2; Q14DD3; Q8N3B8; Q96NH6; Q9HCL1; Q9NWC6;
UniGene Hs.108049;
GeneID 55075
KEGG hsa:55075;
Human Uveal autoantigen with coiled-coil domains and ankyrin repeats Protein SEQUENCE
SEQUENCE 1416 AA; 162505 MW; 92C1104445FB0009 CRC64;
MKSLKSRLRR QDVPGPASSG AAAASAHAAD WNKYDDRLMK AAERGDVEKV
TSILAKKGVN PGKLDVEGRS VFHVVTSKGN LECLNAILIH GVDITTSDTA
GRNALHLAAK YGHALCLQKL LQYNCPTEHA DLQGRTALHD AAMADCPSSI
QLLCDHGASV NAKDVDGRTP LVLATQMSRP TICQLLIDRG ADVNSRDKQN
RTALMLGCEY GCRDAVEVLI KNGADISLLD ALGHDSSYYA RIGDNLDILT
LLKTASENTN KGRELWKKGP SLQQRNLTHM QDEVNVKSHQ REHQNIQDLE
IENEDLKERL RKIQQEQRIL LDKVNGLQLQ LNEEVMVADD LESEREKLKS
LLAAKEKQHE ESLRTIEALK NRFKYFESDH LGSGSHFSNR KEDMLLKQGQ
MYMADSQCTS PGIPAHMQSR SMLRPLELSL PSQTSYSENE ILKKELEAMR
TFCESAKQDR LKLQNELAHK VAECKALALE CERVKEDSDE QIKQLEDALK
DVQKRMYESE GKVKQMQTHF LALKEHLTSE AASGNHRLTE ELKDQLKDLK
VKYEGASAEV GKLRNQIKQN EMIVEEFKRD EGKLIEENKR LQKELSMCEM
EREKKGRKVT EMEGQAKELS AKLALSIPAE KFENMKSSLS NEVNEKAKKL
VEMEREHEKS LSEIRQLKRE LENVKAKLAQ HVKPEEHEQV KSRLEQKSGE
LGKKITELTL KNQTLQKEIE KVYLDNKLLK EQAHNLTIEM KNHYVPLKVS
EDMKKSHDAI IDDLNRKLLD VTQKYTEKKL EMEKLLLEND SLSKDVSRLE
TVFVPPEKHE KEIIALKSNI VELKKQLSEL KKKCGEDQEK IHALTSENTN
LKKMMSNQYV PVKTHEEVKM TLNDTLAKTN RELLDVKKKF EDINQEFVKI
KDKNEILKRN LENTQNQIKA EYISLAEHEA KMSSLSQSMR KVQDSNAEIL
ANYRKGQEEI VTLHAEIKAQ KKELDTIQEC IKVKYAPIVS FEECERKFKA
TEKELKDQLS EQTQKYSVSE EEVKKNKQEN DKLKKEIFTL QKDLRDKTVL
IEKSHEMERA LSRKTDELNK QLKDLSQKYT EVKNVKEKLV EENAKQTSEI
LAVQNLLQKQ HVPLEQVEAL KKSLNGTIEN LKEELKSMQR CYEKEQQTVT
KLHQLLENQK NSSVPLAEHL QIKEAFEKEV GIIKASLREK EEESQNKMEE
VSKLQSEVQN TKQALKKLET REVVDLSKYK ATKSDLETQI SSLNEKLANL
NRKYEEVCEE VLHAKKKEIS AKDEKELLHF SIEQEIKDQK ERCDKSLTTI
TELQRRIQES AKQIEAKDNK ITELLNDVER LKQALNGLSQ LTYTSGNPTK
RQSQLIDTLQ HQVKSLEQQL ADADRQHQEV IAIYRTHLLS AAQGHMDEDV
QEALLQIIQM RQGLVC
UCSC uc002asq.4; human. [Q9BZF9-1];

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Category

Human UACA ELISA Kit

Human KIAA1561 ELISA Kit;Human NUCLING ELISA Kit;Human uveal autoantigen with coiled-coil domains and ankyrin repeats ELISA Kit;Human nuclear membrane binding protein ELISA Kit;