Human UACA ELISA Kit

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  • Alternative name

    Human KIAA1561 ELISA Kit;Human NUCLING ELISA Kit;Human uveal autoantigen with coiled-coil domains and ankyrin repeats ELISA Kit;Human nuclear membrane binding protein ELISA Kit;

  • Catalog
    E002718
  • species
    Human
  • GeneUACA
  • Standard CurveHuman UACA ELISA Kit
  • Other Species Mouse Uaca ELISA KitBovine UACA ELISA KitCanine UACA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of anti-UACA. No significant cross-reactivity or interference between anti-UACA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between anti-UACA and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman UACA ELISA Kit allows for the in vitro quantitative determination of UACA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: Anti-UACA ELISA kit applies the competitive enzyme immunoassay technique utilizing a UACA antigen and an anti-UACA-HRP conjugate. The assay sample and buffer are incubated together with anti-UACA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-UACA concentration since anti-UACA from samples and anti-UACA-HRP conjugate compete for the UACA antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-UACA from the sample, fewer sites are left to bind anti-UACA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-UACA concentration in each sample is interpolated from this standard curve.
  • Human Uveal autoantigen with coiled-coil domains and ankyrin repeats Protein information
  • Uniprot ID UACA_HUMAN
  • Uniprot AC Q9BZF9; G3XAG2; Q14DD3; Q8N3B8; Q96NH6; Q9HCL1; Q9NWC6;
  • UniGene Hs.108049;
  • GeneID 55075
  • KEGG hsa:55075;
  • Human Uveal autoantigen with coiled-coil domains and ankyrin repeats Protein SEQUENCE
  • SEQUENCE 1416 AA; 162505 MW; 92C1104445FB0009 CRC64;

    MKSLKSRLRR QDVPGPASSG AAAASAHAAD WNKYDDRLMK AAERGDVEKV

    TSILAKKGVN PGKLDVEGRS VFHVVTSKGN LECLNAILIH GVDITTSDTA

    GRNALHLAAK YGHALCLQKL LQYNCPTEHA DLQGRTALHD AAMADCPSSI

    QLLCDHGASV NAKDVDGRTP LVLATQMSRP TICQLLIDRG ADVNSRDKQN

    RTALMLGCEY GCRDAVEVLI KNGADISLLD ALGHDSSYYA RIGDNLDILT

    LLKTASENTN KGRELWKKGP SLQQRNLTHM QDEVNVKSHQ REHQNIQDLE

    IENEDLKERL RKIQQEQRIL LDKVNGLQLQ LNEEVMVADD LESEREKLKS

    LLAAKEKQHE ESLRTIEALK NRFKYFESDH LGSGSHFSNR KEDMLLKQGQ

    MYMADSQCTS PGIPAHMQSR SMLRPLELSL PSQTSYSENE ILKKELEAMR

    TFCESAKQDR LKLQNELAHK VAECKALALE CERVKEDSDE QIKQLEDALK

    DVQKRMYESE GKVKQMQTHF LALKEHLTSE AASGNHRLTE ELKDQLKDLK

    VKYEGASAEV GKLRNQIKQN EMIVEEFKRD EGKLIEENKR LQKELSMCEM

    EREKKGRKVT EMEGQAKELS AKLALSIPAE KFENMKSSLS NEVNEKAKKL

    VEMEREHEKS LSEIRQLKRE LENVKAKLAQ HVKPEEHEQV KSRLEQKSGE

    LGKKITELTL KNQTLQKEIE KVYLDNKLLK EQAHNLTIEM KNHYVPLKVS

    EDMKKSHDAI IDDLNRKLLD VTQKYTEKKL EMEKLLLEND SLSKDVSRLE

    TVFVPPEKHE KEIIALKSNI VELKKQLSEL KKKCGEDQEK IHALTSENTN

    LKKMMSNQYV PVKTHEEVKM TLNDTLAKTN RELLDVKKKF EDINQEFVKI

    KDKNEILKRN LENTQNQIKA EYISLAEHEA KMSSLSQSMR KVQDSNAEIL

    ANYRKGQEEI VTLHAEIKAQ KKELDTIQEC IKVKYAPIVS FEECERKFKA

    TEKELKDQLS EQTQKYSVSE EEVKKNKQEN DKLKKEIFTL QKDLRDKTVL

    IEKSHEMERA LSRKTDELNK QLKDLSQKYT EVKNVKEKLV EENAKQTSEI

    LAVQNLLQKQ HVPLEQVEAL KKSLNGTIEN LKEELKSMQR CYEKEQQTVT

    KLHQLLENQK NSSVPLAEHL QIKEAFEKEV GIIKASLREK EEESQNKMEE

    VSKLQSEVQN TKQALKKLET REVVDLSKYK ATKSDLETQI SSLNEKLANL

    NRKYEEVCEE VLHAKKKEIS AKDEKELLHF SIEQEIKDQK ERCDKSLTTI

    TELQRRIQES AKQIEAKDNK ITELLNDVER LKQALNGLSQ LTYTSGNPTK

    RQSQLIDTLQ HQVKSLEQQL ADADRQHQEV IAIYRTHLLS AAQGHMDEDV

    QEALLQIIQM RQGLVC

  • UCSC uc002asq.4; human. [Q9BZF9-1];


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