SpecificityThis assay has high sensitivity and excellent specificity for detection of NFE2R2. No significant cross-reactivity or interference between NFE2R2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFE2R2 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman Nrf2 ELISA Kit allows for the in vitro quantitative determination of Nrf2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: NFE2R2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NFE2R2 antibody and an NFE2R2-HRP conjugate. The assay sample and buffer are incubated together with NFE2R2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFE2R2 concentration since NFE2R2 from samples and NFE2R2-HRP conjugate compete for the anti-NFE2R2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFE2R2 from the sample, fewer sites are left to bind NFE2R2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFE2R2 concentration in each sample is interpolated from this standard curve.