Intended UseMouse Il10 ELISA Kit allows for the in vitro quantitative determination of Il10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: mouse IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-10 has been precoated onto 96-well plates. Standards (E.coli,S19-S178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-10 amount of sample captured in plate.
Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an importantcytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokinewhose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described naturalendogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to thedevelopment of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes tothe heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is animmunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 geneis mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standardproduct used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa.