Mouse Atp1a2 ELISA Kit

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  • Alternative name

    Mouse Na(+)/K(+) ATPase alpha-2 subunit ELISA Kit;Mouse Na(+)/K(+) ATPase alpha(+) subunit ELISA Kit;Mouse sodium pump subunit alpha-2 ELISA Kit;Mouse AW060654 ELISA Kit;Mouse Atpa-3 ELISA Kit;Mouse mKIAA0778 ELISA Kit;Mouse ATPase, Na+/K+ transporting, alpha 2 polypeptide ELISA Kit;Mouse sodium/potassium-transporting ATPase subunit alpha-2 ELISA Kit;

  • Catalog
    E045252
  • species
    Mouse
  • GeneAtp1a2
  • Standard CurveMouse Atp1a2 ELISA Kit
  • Other Species Human ATP1A2 ELISA KitBovine ATP1A2 ELISA KitChicken ATP1A2 ELISA KitPorcine ATP1A2 ELISA KitRat Atp1a2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.042ng/mL.
  • Intended UseMouse Atp1a2 ELISA Kit allows for the in vitro quantitative determination of Atp1a2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Sodium/potassium-transporting ATPase subunit alpha-2 Protein information
  • Uniprot ID AT1A2_MOUSE
  • Uniprot AC Q6PIE5; Q80UZ8;
  • UniGene Mm.207432;
  • GeneID 98660
  • KEGG mmu:98660;
  • Mouse Sodium/potassium-transporting ATPase subunit alpha-2 Protein SEQUENCE
  • SEQUENCE 1020 AA; 112217 MW; 5436E795BD5B4CFA CRC64;

    MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG

    RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI

    LLWIGALLCF LAYGILAAME DEPSNDNLYL GIVLAAVVIV TGCFSYYQEA

    KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD

    LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG

    TARGIVIATG DRTVMGRIAT LASGLEVGQT PIAMEIEHFI QLITGVAVFL

    GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA

    RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT

    TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS

    ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDSPQSH

    VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM ELGGLGERVL

    GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV

    GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS

    QVNPREAKAC VVHGSDLKDM TSEQLDEILR DHTEIVFART SPQQKLIIVE

    GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGISGSDVSK QAADMILLDD

    NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG

    TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK LVNERLISMA

    YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TTNDLEDSYG

    QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI

    LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE

    VRKLILRRYP GGWVEKETYY

  • UCSC uc007dqc.1; mouse.;


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