Mouse Atp1a2 ELISA Kit

Alternative name
Mouse Na(+)/K(+) ATPase alpha-2 subunit ELISA Kit;Mouse Na(+)/K(+) ATPase alpha(+) subunit ELISA Kit;Mouse sodium pump subunit alpha-2 ELISA Kit;Mouse AW060654 ELISA Kit;Mouse Atpa-3 ELISA Kit;Mouse mKIAA0778 ELISA Kit;Mouse ATPase, Na+/K+ transporting, alpha 2 polypeptide ELISA Kit;Mouse sodium/potassium-transporting ATPase subunit alpha-2 ELISA Kit;
Catalog
E045252
species
Mouse
GeneAtp1a2
Standard Curve
Other Species Human ATP1A2 ELISA Kit Bovine ATP1A2 ELISA Kit Chicken ATP1A2 ELISA Kit Porcine ATP1A2 ELISA Kit Rat Atp1a2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.042ng/mL.
Intended UseMouse Atp1a2 ELISA Kit allows for the in vitro quantitative determination of Atp1a2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Sodium/potassium-transporting ATPase subunit alpha-2 Protein information
Uniprot ID AT1A2_MOUSE
Uniprot AC Q6PIE5; Q80UZ8;
UniGene Mm.207432;
GeneID 98660
KEGG mmu:98660;
Mouse Sodium/potassium-transporting ATPase subunit alpha-2 Protein SEQUENCE
SEQUENCE 1020 AA; 112217 MW; 5436E795BD5B4CFA CRC64;
MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG
RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI
LLWIGALLCF LAYGILAAME DEPSNDNLYL GIVLAAVVIV TGCFSYYQEA
KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD
LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG
TARGIVIATG DRTVMGRIAT LASGLEVGQT PIAMEIEHFI QLITGVAVFL
GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT
TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS
ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDSPQSH
VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM ELGGLGERVL
GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV
GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS
QVNPREAKAC VVHGSDLKDM TSEQLDEILR DHTEIVFART SPQQKLIIVE
GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGISGSDVSK QAADMILLDD
NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG
TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK LVNERLISMA
YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TTNDLEDSYG
QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI
LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE
VRKLILRRYP GGWVEKETYY
UCSC uc007dqc.1; mouse.;

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Mouse Atp1a2 ELISA Kit