Human NPC1 ELISA Kit

Alternative name
Human NPC ELISA Kit;Human NPC intracellular cholesterol transporter 1 ELISA Kit;Human Niemann-Pick C1 protein ELISA Kit;Human truncated Niemann-Pick C1 ELISA Kit;
Catalog
E026563
species
Human
GeneNPC1
SpecificityThis assay has high sensitivity and excellent specificity for detection of NPC1. No significant cross-reactivity or interference between NPC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NPC1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman NPC1 ELISA Kit allows for the in vitro quantitative determination of NPC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: NPC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NPC1 antibody and an NPC1-HRP conjugate. The assay sample and buffer are incubated together with NPC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPC1 concentration since NPC1 from samples and NPC1-HRP conjugate compete for the anti-NPC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NPC1 from the sample, fewer sites are left to bind NPC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPC1 concentration in each sample is interpolated from this standard curve.
Human Niemann-Pick C1 protein Protein information
Uniprot ID NPC1_HUMAN
Uniprot AC O15118; B4DET3; Q9P130;
UniGene Hs.464779; Hs.715623;
GeneID 4864
KEGG hsa:4864;
Human Niemann-Pick C1 protein Protein SEQUENCE
SEQUENCE 1278 AA; 142167 MW; DA1523E09822E5C7 CRC64;
MTARGLALGL LLLLLCPAQV FSQSCVWYGE CGIAYGDKRY NCEYSGPPKP
LPKDGYDLVQ ELCPGFFFGN VSLCCDVRQL QTLKDNLQLP LQFLSRCPSC
FYNLLNLFCE LTCSPRQSQF LNVTATEDYV DPVTNQTKTN VKELQYYVGQ
SFANAMYNAC RDVEAPSSND KALGLLCGKD ADACNATNWI EYMFNKDNGQ
APFTITPVFS DFPVHGMEPM NNATKGCDES VDEVTAPCSC QDCSIVCGPK
PQPPPPPAPW TILGLDAMYV IMWITYMAFL LVFFGAFFAV WCYRKRYFVS
EYTPIDSNIA FSVNASDKGE ASCCDPVSAA FEGCLRRLFT RWGSFCVRNP
GCVIFFSLVF ITACSSGLVF VRVTTNPVDL WSAPSSQARL EKEYFDQHFG
PFFRTEQLII RAPLTDKHIY QPYPSGADVP FGPPLDIQIL HQVLDLQIAI
ENITASYDNE TVTLQDICLA PLSPYNTNCT ILSVLNYFQN SHSVLDHKKG
DDFFVYADYH THFLYCVRAP ASLNDTSLLH DPCLGTFGGP VFPWLVLGGY
DDQNYNNATA LVITFPVNNY YNDTEKLQRA QAWEKEFINF VKNYKNPNLT
ISFTAERSIE DELNRESDSD VFTVVISYAI MFLYISLALG HMKSCRRLLV
DSKVSLGIAG ILIVLSSVAC SLGVFSYIGL PLTLIVIEVI PFLVLAVGVD
NIFILVQAYQ RDERLQGETL DQQLGRVLGE VAPSMFLSSF SETVAFFLGA
LSVMPAVHTF SLFAGLAVFI DFLLQITCFV SLLGLDIKRQ EKNRLDIFCC
VRGAEDGTSV QASESCLFRF FKNSYSPLLL KDWMRPIVIA IFVGVLSFSI
AVLNKVDIGL DQSLSMPDDS YMVDYFKSIS QYLHAGPPVY FVLEEGHDYT
SSKGQNMVCG GMGCNNDSLV QQIFNAAQLD NYTRIGFAPS SWIDDYFDWV
KPQSSCCRVD NITDQFCNAS VVDPACVRCR PLTPEGKQRP QGGDFMRFLP
MFLSDNPNPK CGKGGHAAYS SAVNILLGHG TRVGATYFMT YHTVLQTSAD
FIDALKKARL IASNVTETMG INGSAYRVFP YSVFYVFYEQ YLTIIDDTIF
NLGVSLGAIF LVTMVLLGCE LWSAVIMCAT IAMVLVNMFG VMWLWGISLN
AVSLVNLVMS CGISVEFCSH ITRAFTVSMK GSRVERAEEA LAHMGSSVFS
GITLTKFGGI VVLAFAKSQI FQIFYFRMYL AMVLLGATHG LIFLPVLLSY
IGPSVNKAKS CATEERYKGT ERERLLNF
UCSC uc002kum.5; human. [O15118-1];

Category

Human NPC1 ELISA Kit

Human NPC ELISA Kit;Human NPC intracellular cholesterol transporter 1 ELISA Kit;Human Niemann-Pick C1 protein ELISA Kit;Human truncated Niemann-Pick C1 ELISA Kit;