Human NPC1 ELISA Kit

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  • Alternative name

    Human NPC ELISA Kit;Human NPC intracellular cholesterol transporter 1 ELISA Kit;Human Niemann-Pick C1 protein ELISA Kit;Human truncated Niemann-Pick C1 ELISA Kit;

  • Catalog
    E026563
  • species
    Human
  • GeneNPC1
  • Standard CurveHuman NPC1 ELISA Kit
  • Other Species Mouse Npc1 ELISA KitPorcine NPC1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NPC1. No significant cross-reactivity or interference between NPC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NPC1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman NPC1 ELISA Kit allows for the in vitro quantitative determination of NPC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: NPC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NPC1 antibody and an NPC1-HRP conjugate. The assay sample and buffer are incubated together with NPC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPC1 concentration since NPC1 from samples and NPC1-HRP conjugate compete for the anti-NPC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NPC1 from the sample, fewer sites are left to bind NPC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPC1 concentration in each sample is interpolated from this standard curve.
  • Human Niemann-Pick C1 protein Protein information
  • Uniprot ID NPC1_HUMAN
  • Uniprot AC O15118; B4DET3; Q9P130;
  • UniGene Hs.464779; Hs.715623;
  • GeneID 4864
  • KEGG hsa:4864;
  • Human Niemann-Pick C1 protein Protein SEQUENCE
  • SEQUENCE 1278 AA; 142167 MW; DA1523E09822E5C7 CRC64;

    MTARGLALGL LLLLLCPAQV FSQSCVWYGE CGIAYGDKRY NCEYSGPPKP

    LPKDGYDLVQ ELCPGFFFGN VSLCCDVRQL QTLKDNLQLP LQFLSRCPSC

    FYNLLNLFCE LTCSPRQSQF LNVTATEDYV DPVTNQTKTN VKELQYYVGQ

    SFANAMYNAC RDVEAPSSND KALGLLCGKD ADACNATNWI EYMFNKDNGQ

    APFTITPVFS DFPVHGMEPM NNATKGCDES VDEVTAPCSC QDCSIVCGPK

    PQPPPPPAPW TILGLDAMYV IMWITYMAFL LVFFGAFFAV WCYRKRYFVS

    EYTPIDSNIA FSVNASDKGE ASCCDPVSAA FEGCLRRLFT RWGSFCVRNP

    GCVIFFSLVF ITACSSGLVF VRVTTNPVDL WSAPSSQARL EKEYFDQHFG

    PFFRTEQLII RAPLTDKHIY QPYPSGADVP FGPPLDIQIL HQVLDLQIAI

    ENITASYDNE TVTLQDICLA PLSPYNTNCT ILSVLNYFQN SHSVLDHKKG

    DDFFVYADYH THFLYCVRAP ASLNDTSLLH DPCLGTFGGP VFPWLVLGGY

    DDQNYNNATA LVITFPVNNY YNDTEKLQRA QAWEKEFINF VKNYKNPNLT

    ISFTAERSIE DELNRESDSD VFTVVISYAI MFLYISLALG HMKSCRRLLV

    DSKVSLGIAG ILIVLSSVAC SLGVFSYIGL PLTLIVIEVI PFLVLAVGVD

    NIFILVQAYQ RDERLQGETL DQQLGRVLGE VAPSMFLSSF SETVAFFLGA

    LSVMPAVHTF SLFAGLAVFI DFLLQITCFV SLLGLDIKRQ EKNRLDIFCC

    VRGAEDGTSV QASESCLFRF FKNSYSPLLL KDWMRPIVIA IFVGVLSFSI

    AVLNKVDIGL DQSLSMPDDS YMVDYFKSIS QYLHAGPPVY FVLEEGHDYT

    SSKGQNMVCG GMGCNNDSLV QQIFNAAQLD NYTRIGFAPS SWIDDYFDWV

    KPQSSCCRVD NITDQFCNAS VVDPACVRCR PLTPEGKQRP QGGDFMRFLP

    MFLSDNPNPK CGKGGHAAYS SAVNILLGHG TRVGATYFMT YHTVLQTSAD

    FIDALKKARL IASNVTETMG INGSAYRVFP YSVFYVFYEQ YLTIIDDTIF

    NLGVSLGAIF LVTMVLLGCE LWSAVIMCAT IAMVLVNMFG VMWLWGISLN

    AVSLVNLVMS CGISVEFCSH ITRAFTVSMK GSRVERAEEA LAHMGSSVFS

    GITLTKFGGI VVLAFAKSQI FQIFYFRMYL AMVLLGATHG LIFLPVLLSY

    IGPSVNKAKS CATEERYKGT ERERLLNF

  • UCSC uc002kum.5; human. [O15118-1];


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