Human DRD1 ELISA Kit

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  • Alternative name

    Human dopamine D1 receptor ELISA Kit;Human DADR ELISA Kit;Human DRD1A ELISA Kit;Human dopamine receptor D1 ELISA Kit;Human D(1A) dopamine receptor ELISA Kit;

  • Catalog
    E012307
  • species
    Human
  • GeneDRD1
  • Other Species Human D1R/DRD1 ELISA KitMouse Drd1 ELISA KitBovine DRD1 ELISA KitPorcine DRD1 ELISA KitRabbit DRD1 ELISA KitRat Drd1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DRD1. No significant cross-reactivity or interference between DRD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DRD1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman DRD1 ELISA Kit allows for the in vitro quantitative determination of DRD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This DRD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DRD1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||DRD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DRD1 antibody and an DRD1-HRP conjugate. The assay sample and buffer are incubated together with DRD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD1 concentration since DRD1 from samples and DRD1-HRP conjugate compete for the anti-DRD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD1 from the sample, fewer sites are left to bind DRD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD1 concentration in each sample is interpolated from this standard curve.
  • Human D(1A) dopamine receptor Protein information
  • Uniprot ID DRD1_HUMAN
  • Uniprot AC P21728; B2RA44; Q4QRJ0;
  • UniGene Hs.2624;
  • GeneID 1812
  • KEGG hsa:1812;
  • Human D(1A) dopamine receptor Protein SEQUENCE
  • SEQUENCE 446 AA; 49293 MW; 64E062D765D0DBA7 CRC64;

    MRTLNTSAMD GTGLVVERDF SVRILTACFL SLLILSTLLG NTLVCAAVIR

    FRHLRSKVTN FFVISLAVSD LLVAVLVMPW KAVAEIAGFW PFGSFCNIWV

    AFDIMCSTAS ILNLCVISVD RYWAISSPFR YERKMTPKAA FILISVAWTL

    SVLISFIPVQ LSWHKAKPTS PSDGNATSLA ETIDNCDSSL SRTYAISSSV

    ISFYIPVAIM IVTYTRIYRI AQKQIRRIAA LERAAVHAKN CQTTTGNGKP

    VECSQPESSF KMSFKRETKV LKTLSVIMGV FVCCWLPFFI LNCILPFCGS

    GETQPFCIDS NTFDVFVWFG WANSSLNPII YAFNADFRKA FSTLLGCYRL

    CPATNNAIET VSINNNGAAM FSSHHEPRGS ISKECNLVYL IPHAVGSSED

    LKKEEAAGIA RPLEKLSPAL SVILDYDTDV SLEKIQPITQ NGQHPT

  • UCSC uc003mcz.4; human.;



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