Human DRD1 ELISA Kit

Alternative name
Human dopamine D1 receptor ELISA Kit;Human DADR ELISA Kit;Human DRD1A ELISA Kit;Human dopamine receptor D1 ELISA Kit;Human D(1A) dopamine receptor ELISA Kit;
Catalog
E012307
species
Human
GeneDRD1
SpecificityThis assay has high sensitivity and excellent specificity for detection of DRD1. No significant cross-reactivity or interference between DRD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DRD1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman DRD1 ELISA Kit allows for the in vitro quantitative determination of DRD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This DRD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DRD1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||DRD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DRD1 antibody and an DRD1-HRP conjugate. The assay sample and buffer are incubated together with DRD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD1 concentration since DRD1 from samples and DRD1-HRP conjugate compete for the anti-DRD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD1 from the sample, fewer sites are left to bind DRD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD1 concentration in each sample is interpolated from this standard curve.
Human D(1A) dopamine receptor Protein information
Uniprot ID DRD1_HUMAN
Uniprot AC P21728; B2RA44; Q4QRJ0;
UniGene Hs.2624;
GeneID 1812
KEGG hsa:1812;
Human D(1A) dopamine receptor Protein SEQUENCE
SEQUENCE 446 AA; 49293 MW; 64E062D765D0DBA7 CRC64;
MRTLNTSAMD GTGLVVERDF SVRILTACFL SLLILSTLLG NTLVCAAVIR
FRHLRSKVTN FFVISLAVSD LLVAVLVMPW KAVAEIAGFW PFGSFCNIWV
AFDIMCSTAS ILNLCVISVD RYWAISSPFR YERKMTPKAA FILISVAWTL
SVLISFIPVQ LSWHKAKPTS PSDGNATSLA ETIDNCDSSL SRTYAISSSV
ISFYIPVAIM IVTYTRIYRI AQKQIRRIAA LERAAVHAKN CQTTTGNGKP
VECSQPESSF KMSFKRETKV LKTLSVIMGV FVCCWLPFFI LNCILPFCGS
GETQPFCIDS NTFDVFVWFG WANSSLNPII YAFNADFRKA FSTLLGCYRL
CPATNNAIET VSINNNGAAM FSSHHEPRGS ISKECNLVYL IPHAVGSSED
LKKEEAAGIA RPLEKLSPAL SVILDYDTDV SLEKIQPITQ NGQHPT
UCSC uc003mcz.4; human.;

Category

Human DRD1 ELISA Kit

Human dopamine D1 receptor ELISA Kit;Human DADR ELISA Kit;Human DRD1A ELISA Kit;Human dopamine receptor D1 ELISA Kit;Human D(1A) dopamine receptor ELISA Kit;