Human NAMPT ELISA Kit

Alternative name
Human NAmPRTase ELISA Kit;Human Nampt ELISA Kit;Human pre-B-cell colony-enhancing factor 1 ELISA Kit;Human pre-B cell-enhancing factor ELISA Kit;Human VISFATIN ELISA Kit;Human PBEF ELISA Kit;Human PBEF1 ELISA Kit;Human 1110035O14Rik ELISA Kit;Human VF ELISA Kit;Human nicotinamide phosphoribosyltransferase ELISA Kit;
Catalog
E026540
species
Human
GeneNAMPT
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NAMPT. No significant cross-reactivity or interference between Human NAMPT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.156 ng/ml
Intended UseHuman NAMPT ELISA Kit allows for the in vitro quantitative determination of NAMPT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalFor SamplesFor the quantitative determination of human visfatin concentrations in serum, plasma, urine, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for visfatin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any visfatin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for visfatin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of visfatin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Nicotinamide phosphoribosyltransferase Protein information
Uniprot ID NAMPT_HUMAN
Uniprot AC P43490; A4D0Q9; A4D0R0; Q3KQV0; Q8WW95;
UniGene Hs.489615;
GeneID 10135
KEGG hsa:10135;
Human Nicotinamide phosphoribosyltransferase Protein SEQUENCE
SEQUENCE 491 AA; 55521 MW; 6BF07B631B589AC0 CRC64;
MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR
KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKG
WNYILEKYDG HLPIEIKAVP EGFVIPRGNV LFTVENTDPE CYWLTNWIET
ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS
QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY SVPAAEHSTI
TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV
SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKG YKLLPPYLRV
IQGDGVDINT LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK
CSYVVTNGLG INVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL
EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH H
UCSC uc003vdq.4; human.;

Category

Human NAMPT ELISA Kit