Mouse NT3 ELISA Kit

Contact Us
  • Alternative name

    Neurotrophin-3 ELISA KIT; HDNF ELISA KIT; Nerve growth factor 2 ELISA KIT; NGF-2 ELISA KIT; Neurotrophic factorNTF3 ELISA KIT; NT-3 ELISA KIT; NGF-2 ELISA KIT

  • Catalog
    E061527
  • species
    Mouse
  • GeneNT3
  • Standard CurveMouse NT3 ELISA Kit
  • Other Species Human NT3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Neurotrophin 3 (NT3). No significant cross-reactivity or interference between Neurotrophin 3 (NT3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 33pg/mL.
  • Intended UseMouse NT3 ELISA Kit allows for the in vitro quantitative determination of NT3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotrophin 3 (NT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotrophin 3 (NT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotrophin 3 (NT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurotrophin 3 (NT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



Inquiry About Mouse NT3 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!