Human C8G ELISA Kit

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  • Alternative name

    Human C8C ELISA Kit;Human complement C8 gamma chain ELISA Kit;Human complement component C8 gamma chain ELISA Kit;Human complement component 8, gamma polypeptide ELISA Kit;

  • Catalog
    E005787
  • species
    Human
  • GeneC8G
  • Other Species Mouse C8g ELISA KitRabbit C8G ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human C8g. No significant cross-reactivity or interference between Human C8g and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human C8g and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman C8G ELISA Kit allows for the in vitro quantitative determination of C8G , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C8g in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C8g. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human C8g and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C8g, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human C8g. You can calculate the concentration of Human C8g in the samples by comparing the OD of the samples to the standard curve.
  • Human Complement component C8 gamma chain Protein information
  • Uniprot ID CO8G_HUMAN
  • Uniprot AC P07360; Q14CT8; Q14CU0; Q5SQ07;
  • UniGene Hs.1285;
  • GeneID 733
  • KEGG hsa:733;
  • Human Complement component C8 gamma chain Protein SEQUENCE
  • SEQUENCE 202 AA; 22277 MW; 4E4F73140E607FFE CRC64;

    MLPPGTATLL TLLLAAGSLG QKPQRPRRPA SPISTIQPKA NFDAQQFAGT

    WLLVAVGSAC RFLQEQGHRA EATTLHVAPQ GTAMAVSTFR KLDGICWQVR

    QLYGDTGVLG RFLLQARDAR GAVHVVVAET DYQSFAVLYL ERAGQLSVKL

    YARSLPVSDS VLSGFEQRVQ EAHLTEDQIF YFPKYGFCEA ADQFHVLDEV

    RR

  • UCSC uc004cka.3; human.;


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