Human NEXN ELISA Kit

Alternative name
Human F-actin-binding protein ELISA Kit;Human NELIN ELISA Kit;Human CMH20 ELISA Kit;Human nexilin F-actin binding protein ELISA Kit;Human nexilin ELISA Kit;
Catalog
E026446
species
Human
GeneNEXN
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nexilin (NEXN). No significant cross-reactivity or interference between Nexilin (NEXN) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 6.4pg/mL.
Intended UseHuman NEXN ELISA Kit allows for the in vitro quantitative determination of NEXN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nexilin (NEXN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nexilin (NEXN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nexilin (NEXN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nexilin (NEXN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Nexilin Protein information
Uniprot ID NEXN_HUMAN
Uniprot AC Q96DL0; Q9Y2V1;
UniGene Hs.612385;
GeneID 91624
KEGG hsa:91624;
Human Nexilin Protein SEQUENCE
SEQUENCE 675 AA; 80658 MW; 18D6336E62503E7F CRC64;
MNDISQKAEI LLSSSKPVPK TYVPKLGKGD VKDKFEAMQR AREERNQRRS
RDEKQRRKEQ YIREREWNRR KQEIKEMLAS DDEEDVSSKV EKAYVPKLTG
TVKGRFAEME KQRQEEQRKR TEEERKRRIE QDMLEKRKIQ RELAKRAEQI
EDINNTGTES ASEEGDDSLL ITVVPVKSYK TSGKMKKNFE DLEKEREEKE
RIKYEEDKRI RYEEQRPSLK EAKCLSLVMD DEIESEAKKE SLSPGKLKLT
FEELERQRQE NRKKQAEEEA RKRLEEEKRA FEEARRQMVN EDEENQDTAK
IFKGYRPGKL KLSFEEMERQ RREDEKRKAE EEARRRIEEE KKAFAEARRN
MVVDDDSPEM YKTISQEFLT PGKLEINFEE LLKQKMEEEK RRTEEERKHK
LEMEKQEFEQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS
KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA
RKSEAPFTHK VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA
LQKKREEEEE EEGSIMNGST AEDEEQTRSG APWFKKPLKN TSVVDSEPVR
FTVKVTGEPK PEITWWFEGE ILQDGEDYQY IERGETYCLY LPETFPEDGG
EYMCKAVNNK GSAASTCILT IESKN
UCSC uc001dib.5; human. [Q0ZGT2-1];

Category

Human NEXN ELISA Kit

Human F-actin-binding protein ELISA Kit;Human NELIN ELISA Kit;Human CMH20 ELISA Kit;Human nexilin F-actin binding protein ELISA Kit;Human nexilin ELISA Kit;