Human NEXN ELISA Kit

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  • Alternative name

    Human F-actin-binding protein ELISA Kit;Human NELIN ELISA Kit;Human CMH20 ELISA Kit;Human nexilin F-actin binding protein ELISA Kit;Human nexilin ELISA Kit;

  • Catalog
    E026446
  • species
    Human
  • GeneNEXN
  • Standard CurveHuman NEXN ELISA Kit
  • Other Species Mouse Nexn ELISA KitHuman NEXN-AS1 ELISA KitRat Nexn ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nexilin (NEXN). No significant cross-reactivity or interference between Nexilin (NEXN) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.4pg/mL.
  • Intended UseHuman NEXN ELISA Kit allows for the in vitro quantitative determination of NEXN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nexilin (NEXN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nexilin (NEXN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nexilin (NEXN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nexilin (NEXN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nexilin Protein information
  • Uniprot ID NEXN_HUMAN
  • Uniprot AC Q96DL0; Q9Y2V1;
  • UniGene Hs.612385;
  • GeneID 91624
  • KEGG hsa:91624;
  • Human Nexilin Protein SEQUENCE
  • SEQUENCE 675 AA; 80658 MW; 18D6336E62503E7F CRC64;

    MNDISQKAEI LLSSSKPVPK TYVPKLGKGD VKDKFEAMQR AREERNQRRS

    RDEKQRRKEQ YIREREWNRR KQEIKEMLAS DDEEDVSSKV EKAYVPKLTG

    TVKGRFAEME KQRQEEQRKR TEEERKRRIE QDMLEKRKIQ RELAKRAEQI

    EDINNTGTES ASEEGDDSLL ITVVPVKSYK TSGKMKKNFE DLEKEREEKE

    RIKYEEDKRI RYEEQRPSLK EAKCLSLVMD DEIESEAKKE SLSPGKLKLT

    FEELERQRQE NRKKQAEEEA RKRLEEEKRA FEEARRQMVN EDEENQDTAK

    IFKGYRPGKL KLSFEEMERQ RREDEKRKAE EEARRRIEEE KKAFAEARRN

    MVVDDDSPEM YKTISQEFLT PGKLEINFEE LLKQKMEEEK RRTEEERKHK

    LEMEKQEFEQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS

    KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA

    RKSEAPFTHK VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA

    LQKKREEEEE EEGSIMNGST AEDEEQTRSG APWFKKPLKN TSVVDSEPVR

    FTVKVTGEPK PEITWWFEGE ILQDGEDYQY IERGETYCLY LPETFPEDGG

    EYMCKAVNNK GSAASTCILT IESKN

  • UCSC uc001dib.5; human. [Q0ZGT2-1];


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