Human JAK2 ELISA Kit

Alternative name
Human Janus kinase 2 ELISA Kit;Human JAK-2 ELISA Kit;Human JTK10 ELISA Kit;Human THCYT3 ELISA Kit;Human tyrosine-protein kinase JAK2 ELISA Kit;Human Janus kinase 2 (a protein tyrosine kinase) ELISA Kit;
Catalog
E040161
species
Human
GeneJAK2
SpecificityThis assay has high sensitivity and excellent specificity for detection of JAK2. No significant cross-reactivity or interference between JAK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between JAK2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman JAK2 ELISA Kit allows for the in vitro quantitative determination of JAK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This JAK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human JAK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||JAK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-JAK2 antibody and an JAK2-HRP conjugate. The assay sample and buffer are incubated together with JAK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the JAK2 concentration since JAK2 from samples and JAK2-HRP conjugate compete for the anti-JAK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by JAK2 from the sample, fewer sites are left to bind JAK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The JAK2 concentration in each sample is interpolated from this standard curve.
Human Tyrosine-protein kinase JAK2 Protein information
Uniprot ID JAK2_HUMAN
Uniprot AC O60674; O14636; O75297;
UniGene Hs.656213;
GeneID 3717
KEGG hsa:3717;
Human Tyrosine-protein kinase JAK2 Protein SEQUENCE
SEQUENCE 1132 AA; 130674 MW; C30669EF1A7DA80C CRC64;
MGMACLTMTE MEGTSTSSIY QNGDISGNAN SMKQIDPVLQ VYLYHSLGKS
EADYLTFPSG EYVAEEICIA ASKACGITPV YHNMFALMSE TERIWYPPNH
VFHIDESTRH NVLYRIRFYF PRWYCSGSNR AYRHGISRGA EAPLLDDFVM
SYLFAQWRHD FVHGWIKVPV THETQEECLG MAVLDMMRIA KENDQTPLAI
YNSISYKTFL PKCIRAKIQD YHILTRKRIR YRFRRFIQQF SQCKATARNL
KLKYLINLET LQSAFYTEKF EVKEPGSGPS GEEIFATIII TGNGGIQWSR
GKHKESETLT EQDLQLYCDF PNIIDVSIKQ ANQEGSNESR VVTIHKQDGK
NLEIELSSLR EALSFVSLID GYYRLTADAH HYLCKEVAPP AVLENIQSNC
HGPISMDFAI SKLKKAGNQT GLYVLRCSPK DFNKYFLTFA VERENVIEYK
HCLITKNENE EYNLSGTKKN FSSLKDLLNC YQMETVRSDN IIFQFTKCCP
PKPKDKSNLL VFRTNGVSDV PTSPTLQRPT HMNQMVFHKI RNEDLIFNES
LGQGTFTKIF KGVRREVGDY GQLHETEVLL KVLDKAHRNY SESFFEAASM
MSKLSHKHLV LNYGVCVCGD ENILVQEFVK FGSLDTYLKK NKNCINILWK
LEVAKQLAWA MHFLEENTLI HGNVCAKNIL LIREEDRKTG NPPFIKLSDP
GISITVLPKD ILQERIPWVP PECIENPKNL NLATDKWSFG TTLWEICSGG
DKPLSALDSQ RKLQFYEDRH QLPAPKWAEL ANLINNCMDY EPDFRPSFRA
IIRDLNSLFT PDYELLTEND MLPNMRIGAL GFSGAFEDRD PTQFEERHLK
FLQQLGKGNF GSVEMCRYDP LQDNTGEVVA VKKLQHSTEE HLRDFEREIE
ILKSLQHDNI VKYKGVCYSA GRRNLKLIME YLPYGSLRDY LQKHKERIDH
IKLLQYTSQI CKGMEYLGTK RYIHRDLATR NILVENENRV KIGDFGLTKV
LPQDKEYYKV KEPGESPIFW YAPESLTESK FSVASDVWSF GVVLYELFTY
IEKSKSPPAE FMRMIGNDKQ GQMIVFHLIE LLKNNGRLPR PDGCPDEIYM
IMTECWNNNV NQRPSFRDLA LRVDQIRDNM AG
UCSC uc003ziw.3; human.;

Category

Human JAK2 ELISA Kit

Human Janus kinase 2 ELISA Kit;Human JAK-2 ELISA Kit;Human JTK10 ELISA Kit;Human THCYT3 ELISA Kit;Human tyrosine-protein kinase JAK2 ELISA Kit;Human Janus kinase 2 (a protein tyrosine kinase) ELISA Kit;