Human JAK2 ELISA Kit

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  • Alternative name

    Human Janus kinase 2 ELISA Kit;Human JAK-2 ELISA Kit;Human JTK10 ELISA Kit;Human THCYT3 ELISA Kit;Human tyrosine-protein kinase JAK2 ELISA Kit;Human Janus kinase 2 (a protein tyrosine kinase) ELISA Kit;

  • Catalog
    E040161
  • species
    Human
  • GeneJAK2
  • Standard CurveHuman JAK2 ELISA Kit
  • Other Species Mouse Jak2 ELISA KitChicken JAK2 ELISA KitPorcine JAK2 ELISA KitRat Jak2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of JAK2. No significant cross-reactivity or interference between JAK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between JAK2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman JAK2 ELISA Kit allows for the in vitro quantitative determination of JAK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This JAK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human JAK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||JAK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-JAK2 antibody and an JAK2-HRP conjugate. The assay sample and buffer are incubated together with JAK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the JAK2 concentration since JAK2 from samples and JAK2-HRP conjugate compete for the anti-JAK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by JAK2 from the sample, fewer sites are left to bind JAK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The JAK2 concentration in each sample is interpolated from this standard curve.
  • Human Tyrosine-protein kinase JAK2 Protein information
  • Uniprot ID JAK2_HUMAN
  • Uniprot AC O60674; O14636; O75297;
  • UniGene Hs.656213;
  • GeneID 3717
  • KEGG hsa:3717;
  • Human Tyrosine-protein kinase JAK2 Protein SEQUENCE
  • SEQUENCE 1132 AA; 130674 MW; C30669EF1A7DA80C CRC64;

    MGMACLTMTE MEGTSTSSIY QNGDISGNAN SMKQIDPVLQ VYLYHSLGKS

    EADYLTFPSG EYVAEEICIA ASKACGITPV YHNMFALMSE TERIWYPPNH

    VFHIDESTRH NVLYRIRFYF PRWYCSGSNR AYRHGISRGA EAPLLDDFVM

    SYLFAQWRHD FVHGWIKVPV THETQEECLG MAVLDMMRIA KENDQTPLAI

    YNSISYKTFL PKCIRAKIQD YHILTRKRIR YRFRRFIQQF SQCKATARNL

    KLKYLINLET LQSAFYTEKF EVKEPGSGPS GEEIFATIII TGNGGIQWSR

    GKHKESETLT EQDLQLYCDF PNIIDVSIKQ ANQEGSNESR VVTIHKQDGK

    NLEIELSSLR EALSFVSLID GYYRLTADAH HYLCKEVAPP AVLENIQSNC

    HGPISMDFAI SKLKKAGNQT GLYVLRCSPK DFNKYFLTFA VERENVIEYK

    HCLITKNENE EYNLSGTKKN FSSLKDLLNC YQMETVRSDN IIFQFTKCCP

    PKPKDKSNLL VFRTNGVSDV PTSPTLQRPT HMNQMVFHKI RNEDLIFNES

    LGQGTFTKIF KGVRREVGDY GQLHETEVLL KVLDKAHRNY SESFFEAASM

    MSKLSHKHLV LNYGVCVCGD ENILVQEFVK FGSLDTYLKK NKNCINILWK

    LEVAKQLAWA MHFLEENTLI HGNVCAKNIL LIREEDRKTG NPPFIKLSDP

    GISITVLPKD ILQERIPWVP PECIENPKNL NLATDKWSFG TTLWEICSGG

    DKPLSALDSQ RKLQFYEDRH QLPAPKWAEL ANLINNCMDY EPDFRPSFRA

    IIRDLNSLFT PDYELLTEND MLPNMRIGAL GFSGAFEDRD PTQFEERHLK

    FLQQLGKGNF GSVEMCRYDP LQDNTGEVVA VKKLQHSTEE HLRDFEREIE

    ILKSLQHDNI VKYKGVCYSA GRRNLKLIME YLPYGSLRDY LQKHKERIDH

    IKLLQYTSQI CKGMEYLGTK RYIHRDLATR NILVENENRV KIGDFGLTKV

    LPQDKEYYKV KEPGESPIFW YAPESLTESK FSVASDVWSF GVVLYELFTY

    IEKSKSPPAE FMRMIGNDKQ GQMIVFHLIE LLKNNGRLPR PDGCPDEIYM

    IMTECWNNNV NQRPSFRDLA LRVDQIRDNM AG

  • UCSC uc003ziw.3; human.;


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