Mouse IFN-alpha ELISA Kit

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  • Alternative name

    Interferon omega-1 ELISA KIT; Interferon alpha-II-1IFNW1 ELISA KIT;

  • Catalog
  • species
  • GeneIFN-alpha
  • Other Species Human IFN-alpha ELISA KitHuman IFN Alpha ELISA KitHuman IFN-alpha / betaR ELISA KitHuman IFN-alpha/betaR ELISA KitHuman IFN-alpha-AB ELISA KitMouse IFN-alpha-Ab ELISA KitMouse IFN-alpha / betaR ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.4pg/ml
  • Intended UseMouse IFN-alpha ELISA Kit allows for the in vitro quantitative determination of IFN-alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse IFN- alphaantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse IFN-alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IFN-alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse IFN-alphacan be calculated.

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