Human GZMA ELISA Kit

Alternative name
Human CTL tryptase ELISA Kit;Human cytotoxic T-lymphocyte proteinase 1 ELISA Kit;Human fragmentin-1 ELISA Kit;Human Granzyme-1 ELISA Kit;Human Hanukkah factor ELISA Kit;Human h factor ELISA Kit;Human HF ELISA Kit;Human CTLA3 ELISA Kit;Human HFSP ELISA Kit;Human granzyme A ELISA Kit;Human Cytotoxic T-lymphocyte-associated serine esterase-3 ELISA Kit;Human Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease) ELISA Kit;Human Hanukah factor serine protease) ELISA Kit;Human granzyme 1 ELISA Kit;Human granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) ELISA Kit;
Catalog
E017428
species
Human
GeneGZMA
SpecificityThis assay has high sensitivity and excellent specificity for detection of human Gzms-A. No significant cross-reactivity or interference between human Gzms-A and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human Gzms-A is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman GZMA ELISA Kit allows for the in vitro quantitative determination of GZMA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Gzms-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Gzms-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Gzms-A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Gzms-A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Granzyme A Protein information
Uniprot ID GRAA_HUMAN
Uniprot AC P12544; A4PHN1; Q6IB36;
UniGene Hs.90708;
GeneID 3001
KEGG hsa:3001;
Human Granzyme A Protein SEQUENCE
SEQUENCE 262 AA; 28999 MW; FD773628BA6F301B CRC64;
MRNSYRFLAS SLSVVVSLLL IPEDVCEKII GGNEVTPHSR PYMVLLSLDR
KTICAGALIA KDWVLTAAHC NLNKRSQVIL GAHSITREEP TKQIMLVKKE
FPYPCYDPAT REGDLKLLQL MEKAKINKYV TILHLPKKGD DVKPGTMCQV
AGWGRTHNSA SWSDTLREVN ITIIDRKVCN DRNHYNFNPV IGMNMVCAGS
LRGGRDSCNG DSGSPLLCEG VFRGVTSFGL ENKCGDPRGP GVYILLSKKH
LNWIIMTIKG AV
UCSC uc003jpm.4; human. [P12544-1];

Category

Human GZMA ELISA Kit