Human GNRHR ELISA Kit

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  • Alternative name

    Human gnRH receptor ELISA Kit;Human gnRH-R ELISA Kit;Human GRHR ELISA Kit;Human GNRHR1 ELISA Kit;Human HH7 ELISA Kit;Human LHRHR ELISA Kit;Human LRHR ELISA Kit;Human gonadotropin releasing hormone receptor ELISA Kit;Human gonadotropin-releasing hormone receptor ELISA Kit;Human gonadotropin-releasing hormone (type 1) receptor 1 ELISA Kit;Human leutinizing hormone releasing horomone receptor ELISA Kit;Human leutinizing-releasing hormone receptor ELISA Kit;Human luliberin receptor ELISA Kit;Human type I GnRH receptor ELISA Kit;

  • Catalog
    E002599
  • species
    Human
  • GeneGNRHR
  • Standard CurveHuman GNRHR ELISA Kit
  • Other Species Mouse Gnrhr ELISA KitBovine GNRHR ELISA KitCanine GNRHR ELISA KitGuinea Pig GNRHR ELISA KitHorse GNRHR ELISA KitPorcine GNRHR ELISA KitRat Gnrhr ELISA KitSheep GNRHR ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GRHRAb. No significant cross-reactivity or interference between GRHRAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRHRAb and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GNRHR ELISA Kit allows for the in vitro quantitative determination of GNRHR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeuroscience
  • Product Description
    specifical
    Principle of the assay: GRHRAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Gonadotropin Releasing Hormone Receptor antigen and an GRHRAb-HRP conjugate. The assay sample and buffer are incubated together with GRHRAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRHRAb concentration since GRHRAb from samples and GRHRAb-HRP conjugate compete for the Gonadotropin Releasing Hormone Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by GRHRAb from the sample, fewer sites are left to bind GRHRAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRHRAb concentration in each sample is interpolated from this standard curve.
  • Human Gonadotropin-releasing hormone receptor Protein information
  • Uniprot ID GNRHR_HUMAN
  • Uniprot AC P30968; O75793; Q14D13; Q92644;
  • UniGene Hs.407587;
  • GeneID 2798
  • KEGG hsa:2798;
  • Human Gonadotropin-releasing hormone receptor Protein SEQUENCE
  • SEQUENCE 328 AA; 37731 MW; D02B2EA2F4246D7B CRC64;

    MANSASPEQN QNHCSAINNS IPLMQGNLPT LTLSGKIRVT VTFFLFLLSA

    TFNASFLLKL QKWTQKKEKG KKLSRMKLLL KHLTLANLLE TLIVMPLDGM

    WNITVQWYAG ELLCKVLSYL KLFSMYAPAF MMVVISLDRS LAITRPLALK

    SNSKVGQSMV GLAWILSSVF AGPQLYIFRM IHLADSSGQT KVFSQCVTHC

    SFSQWWHQAF YNFFTFSCLF IIPLFIMLIC NAKIIFTLTR VLHQDPHELQ

    LNQSKNNIPR ARLKTLKMTV AFATSFTVCW TPYYVLGIWY WFDPEMLNRL

    SDPVNHFFFL FAFLNPCFDP LIYGYFSL

  • UCSC uc003hdm.4; human. [P30968-1];


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