SpecificityThis assay has high sensitivity and excellent specificity for detection of H3F3A. No significant cross-reactivity or interference between H3F3A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between H3F3A and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman H3F3A ELISA Kit allows for the in vitro quantitative determination of H3F3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description specificalIntended Uses: This H3F3A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human H3F3A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||H3F3A ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-H3F3A antibody and an H3F3A-HRP conjugate. The assay sample and buffer are incubated together with H3F3A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the H3F3A concentration since H3F3A from samples and H3F3A-HRP conjugate compete for the anti-H3F3A antibody binding site. Since the number of sites is limited, as more sites are occupied by H3F3A from the sample, fewer sites are left to bind H3F3A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The H3F3A concentration in each sample is interpolated from this standard curve.