Human GDF15 ELISA Kit

Alternative name
Human GDF-15 ELISA Kit;Human macrophage inhibitory cytokine 1 ELISA Kit;Human MIC-1 ELISA Kit;Human NSAID-activated gene 1 protein ELISA Kit;Human NAG-1 ELISA Kit;Human NSAID-regulated gene 1 protein ELISA Kit;Human NRG-1 ELISA Kit;Human placental TGF-beta ELISA Kit;Human placental bone morphogenetic protein ELISA Kit;Human prostate differentiation factor ELISA Kit;Human MIC1 ELISA Kit;Human PDF ELISA Kit;Human PLAB ELISA Kit;Human PTGFB ELISA Kit;Human growth differentiation factor 15 ELISA Kit;Human growth/differentiation factor 15 ELISA Kit;Human NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1 ELISA Kit;Human PTGF-beta ELISA Kit;
Catalog
E016284
species
Human
GeneGDF15
SpecificityNatural and recombinant human GDF-15
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman GDF15 ELISA Kit allows for the in vitro quantitative determination of GDF15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF-15 has been precoated onto 96-well plates. Standards(CHO,A197-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDF-15 amount of sample captured in plate. Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
Human Growth/differentiation factor 15 Protein information
Uniprot ID GDF15_HUMAN
Uniprot AC Q99988; O14629; P78360; Q9BWA0; Q9NRT0;
UniGene Hs.616962;
GeneID 9518
KEGG hsa:9518;
Human Growth/differentiation factor 15 Protein SEQUENCE
SEQUENCE 308 AA; 34140 MW; 2FF3959021B95238 CRC64;
MPGQELRTVN GSQMLLVLLV LSWLPHGGAL SLAEASRASF PGPSELHSED
SRFRELRKRY EDLLTRLRAN QSWEDSNTDL VPAPAVRILT PEVRLGSGGH
LHLRISRAAL PEGLPEASRL HRALFRLSPT ASRSWDVTRP LRRQLSLARP
QAPALHLRLS PPPSQSDQLL AESSSARPQL ELHLRPQAAR GRRRARARNG
DHCPLGPGRC CRLHTVRASL EDLGWADWVL SPREVQVTMC IGACPSQFRA
ANMHAQIKTS LHRLKPDTVP APCCVPASYN PMVLIQKTDT GVSLQTYDDL
LAKDCHCI
UCSC uc002niv.2; human.;

Category

Human GDF15 ELISA Kit