Human TBX3 ELISA Kit

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  • Alternative name

    Human T-box protein 3 ELISA Kit;Human TBX3-ISO ELISA Kit;Human UMS ELISA Kit;Human XHL ELISA Kit;Human T-box 3 ELISA Kit;Human T-box transcription factor TBX3 ELISA Kit;Human bladder cancer related protein XHL ELISA Kit;

  • Catalog
    E037263
  • species
    Human
  • GeneTBX3
  • Standard CurveHuman TBX3 ELISA Kit
  • Other Species Mouse Tbx3 ELISA KitChicken TBX3 ELISA KitRat Tbx3 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman TBX3 ELISA Kit allows for the in vitro quantitative determination of TBX3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: TBX3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TBX3 antibody and an TBX3-HRP conjugate. The assay sample and buffer are incubated together with TBX3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBX3 concentration since TBX3 from samples and TBX3-HRP conjugate compete for the anti-TBX3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TBX3 from the sample, fewer sites are left to bind TBX3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBX3 concentration in each sample is interpolated from this standard curve.
  • Human T-box transcription factor TBX3 Protein information
  • Uniprot ID TBX3_HUMAN
  • Uniprot AC O15119; Q8TB20; Q9UKF8;
  • UniGene Hs.744016;
  • GeneID 6926
  • KEGG hsa:6926;
  • Human T-box transcription factor TBX3 Protein SEQUENCE
  • SEQUENCE 743 AA; 79389 MW; D5572F9CF871E89F CRC64;

    MSLSMRDPVI PGTSMAYHPF LPHRAPDFAM SAVLGHQPPF FPALTLPPNG

    AAALSLPGAL AKPIMDQLVG AAETGIPFSS LGPQAHLRPL KTMEPEEEVE

    DDPKVHLEAK ELWDQFHKRG TEMVITKSGR RMFPPFKVRC SGLDKKAKYI

    LLMDIIAADD CRYKFHNSRW MVAGKADPEM PKRMYIHPDS PATGEQWMSK

    VVTFHKLKLT NNISDKHGFT LAFPSDHATW QGNYSFGTQT ILNSMHKYQP

    RFHIVRANDI LKLPYSTFRT YLFPETEFIA VTAYQNDKIT QLKIDNNPFA

    KGFRDTGNGR REKRKQLTLQ SMRVFDERHK KENGTSDESS SEQAAFNCFA

    QASSPAASTV GTSNLKDLCP SEGESDAEAE SKEEHGPEAC DAAKISTTTS

    EEPCRDKGSP AVKAHLFAAE RPRDSGRLDK ASPDSRHSPA TISSSTRGLG

    AEERRSPVRE GTAPAKVEEA RALPGKEAFA PLTVQTDAAA AHLAQGPLPG

    LGFAPGLAGQ QFFNGHPLFL HPSQFAMGGA FSSMAAAGMG PLLATVSGAS

    TGVSGLDSTA MASAAAAQGL SGASAATLPF HLQQHVLASQ GLAMSPFGSL

    FPYPYTYMAA AAAASSAAAS SSVHRHPFLN LNTMRPRLRY SPYSIPVPVP

    DGSSLLTTAL PSMAAAAGPL DGKVAALAAS PASVAVDSGS ELNSRSSTLS

    SSSMSLSPKL CAEKEAATSE LQSIQRLVSG LEAKPDRSRS ASP

  • UCSC uc001tvt.2; human. [O15119-1];


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