Human Humanin mitochondrial ELISA Kit;Human HNM ELISA Kit;
Catalog
E032382
species
Human
GeneMT-RNR2
SpecificityThis assay has high sensitivity and excellent specificity for detection of PHP. No significant cross-reactivity or interference between PHP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PHP and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman MT-RNR2 ELISA Kit allows for the in vitro quantitative determination of MT-RNR2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description specificalIntended Uses: This PHP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PHP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: PHP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PHP antibody and an PHP-HRP conjugate. The assay sample and buffer are incubated together with PHP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PHP concentration since PHP from samples and PHP-HRP conjugate compete for the anti-PHP antibody binding site. Since the number of sites is limited, as more sites are occupied by PHP from the sample, fewer sites are left to bind PHP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PHP concentration in each sample is interpolated from this standard curve.