Human C4A ELISA Kit

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  • Alternative name

    Human Acidic complement C4 ELISA Kit;Human C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 ELISA Kit;Human CO4 ELISA Kit;Human CPAMD2 ELISA Kit;Human C4B1 ELISA Kit;Human C4B12 ELISA Kit;Human C4B2 ELISA Kit;Human C4B3 ELISA Kit;Human C4B5 ELISA Kit;Human C4BD ELISA Kit;Human C4B_2 ELISA Kit;Human C4F ELISA Kit;Human CH ELISA Kit;Human CPAMD3 ELISA Kit;Human complement C4B (Chido blood group) ELISA Kit;Human complement C4-B ELISA Kit;Human C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit;Human Chido form of C4 ELISA Kit;Human basic complement C4 ELISA Kit;Human complement C4B1a ELISA Kit;Human complement component 4B (Chido blood group) ELISA Kit;

  • Catalog
    E005768
  • species
    Human
  • GeneC4A
  • Other Species Mouse C4a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human C4a. No significant cross-reactivity or interference between Human C4a and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human C4a and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.938ng/mL
  • Intended UseHuman C4A ELISA Kit allows for the in vitro quantitative determination of C4A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C4a in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C4a. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human C4a and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human C4a. You can calculate the concentration of Human C4a in the samples by comparing the OD of the samples to the standard curve.
  • Human Complement C4 gamma chain Protein information
  • Uniprot ID CO4A_HUMAN
  • Uniprot AC Q6U2F8; Q6U2G0; Q96EG2; Q96SA8; Q9NPK5; Q9UIP5;
  • UniGene Hs.534847; Hs.720022;
  • GeneID 721
  • KEGG hsa:721;
  • Human Complement C4 gamma chain Protein SEQUENCE
  • SEQUENCE 1744 AA; 192785 MW; 9396A4CC4DA3602C CRC64;

    MRLLWGLIWA SSFFTLSLQK PRLLLFSPSV VHLGVPLSVG VQLQDVPRGQ

    VVKGSVFLRN PSRNNVPCSP KVDFTLSSER DFALLSLQVP LKDAKSCGLH

    QLLRGPEVQL VAHSPWLKDS LSRTTNIQGI NLLFSSRRGH LFLQTDQPIY

    NPGQRVRYRV FALDQKMRPS TDTITVMVEN SHGLRVRKKE VYMPSSIFQD

    DFVIPDISEP GTWKISARFS DGLESNSSTQ FEVKKYVLPN FEVKITPGKP

    YILTVPGHLD EMQLDIQARY IYGKPVQGVA YVRFGLLDED GKKTFFRGLE

    SQTKLVNGQS HISLSKAEFQ DALEKLNMGI TDLQGLRLYV AAAIIESPGG

    EMEEAELTSW YFVSSPFSLD LSKTKRHLVP GAPFLLQALV REMSGSPASG

    IPVKVSATVS SPGSVPEVQD IQQNTDGSGQ VSIPIIIPQT ISELQLSVSA

    GSPHPAIARL TVAAPPSGGP GFLSIERPDS RPPRVGDTLN LNLRAVGSGA

    TFSHYYYMIL SRGQIVFMNR EPKRTLTSVS VFVDHHLAPS FYFVAFYYHG

    DHPVANSLRV DVQAGACEGK LELSVDGAKQ YRNGESVKLH LETDSLALVA

    LGALDTALYA AGSKSHKPLN MGKVFEAMNS YDLGCGPGGG DSALQVFQAA

    GLAFSDGDQW TLSRKRLSCP KEKTTRKKRN VNFQKAINEK LGQYASPTAK

    RCCQDGVTRL PMMRSCEQRA ARVQQPDCRE PFLSCCQFAE SLRKKSRDKG

    QAGLQRALEI LQEEDLIDED DIPVRSFFPE NWLWRVETVD RFQILTLWLP

    DSLTTWEIHG LSLSKTKGLC VATPVQLRVF REFHLHLRLP MSVRRFEQLE

    LRPVLYNYLD KNLTVSVHVS PVEGLCLAGG GGLAQQVLVP AGSARPVAFS

    VVPTAAAAVS LKVVARGSFE FPVGDAVSKV LQIEKEGAIH REELVYELNP

    LDHRGRTLEI PGNSDPNMIP DGDFNSYVRV TASDPLDTLG SEGALSPGGV

    ASLLRLPRGC GEQTMIYLAP TLAASRYLDK TEQWSTLPPE TKDHAVDLIQ

    KGYMRIQQFR KADGSYAAWL SRDSSTWLTA FVLKVLSLAQ EQVGGSPEKL

    QETSNWLLSQ QQADGSFQDP CPVLDRSMQG GLVGNDETVA LTAFVTIALH

    HGLAVFQDEG AEPLKQRVEA SISKANSFLG EKASAGLLGA HAAAITAYAL

    TLTKAPVDLL GVAHNNLMAM AQETGDNLYW GSVTGSQSNA VSPTPAPRNP

    SDPMPQAPAL WIETTAYALL HLLLHEGKAE MADQASAWLT RQGSFQGGFR

    STQDTVIALD ALSAYWIASH TTEERGLNVT LSSTGRNGFK SHALQLNNRQ

    IRGLEEELQF SLGSKINVKV GGNSKGTLKV LRTYNVLDMK NTTCQDLQIE

    VTVKGHVEYT MEANEDYEDY EYDELPAKDD PDAPLQPVTP LQLFEGRRNR

    RRREAPKVVE EQESRVHYTV CIWRNGKVGL SGMAIADVTL LSGFHALRAD

    LEKLTSLSDR YVSHFETEGP HVLLYFDSVP TSRECVGFEA VQEVPVGLVQ

    PASATLYDYY NPERRCSVFY GAPSKSRLLA TLCSAEVCQC AEGKCPRQRR

    ALERGLQDED GYRMKFACYY PRVEYGFQVK VLREDSRAAF RLFETKITQV

    LHFTKDVKAA ANQMRNFLVR ASCRLRLEPG KEYLIMGLDG ATYDLEGHPQ

    YLLDSNSWIE EMPSERLCRS TRQRAACAQL NDFLQEYGTQ GCQV

  • UCSC uc011doy.3; human. [P0C0L4-1];



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