Mouse ASH1 ELISA Kit

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  • Alternative name

    Achaete-scute homolog 1 ELISA KIT; Class A basic helix-loop-helix protein 46 ELISA KIT; bHLHa46ASCL1 ELISA KIT; ASH1 ELISA KIT; BHLHA46 ELISA KIT; HASH1 ELISA KIT; ASH-1 ELISA KIT; hASH1 ELISA KIT; bHLHa46 ELISA KIT

  • Catalog
    E042455
  • species
    Mouse
  • GeneASH1
  • Standard CurveMouse ASH1 ELISA Kit
  • Other Species Human ASH1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ASH1. No significant cross-reactivity or interference between ASH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASH1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse ASH1 ELISA Kit allows for the in vitro quantitative determination of ASH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: ASH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ASH1 antibody and an ASH1-HRP conjugate. The assay sample and buffer are incubated together with ASH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASH1 concentration since ASH1 from samples and ASH1-HRP conjugate compete for the anti-ASH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ASH1 from the sample, fewer sites are left to bind ASH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASH1 concentration in each sample is interpolated from this standard curve.



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