Human NDN ELISA Kit

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  • Alternative name

    Human HsT16328 ELISA Kit;Human PWCR ELISA Kit;Human necdin, MAGE family member ELISA Kit;Human necdin ELISA Kit;Human Prader-Willi syndrome chromosome region ELISA Kit;Human necdin homolog ELISA Kit;Human necdin, melanoma antigen (MAGE) family member ELISA Kit;Human necdin-like protein ELISA Kit;

  • Catalog
    E025905
  • species
    Human
  • GeneNDN
  • Standard CurveHuman NDN ELISA Kit
  • Other Species Mouse Ndn ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Necdin (NDN). No significant cross-reactivity or interference between Necdin (NDN) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman NDN ELISA Kit allows for the in vitro quantitative determination of NDN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Necdin (NDN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Necdin (NDN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Necdin (NDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Necdin (NDN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Necdin Protein information
  • Uniprot ID NECD_HUMAN
  • Uniprot AC Q99608; B2R6Z5;
  • UniGene Hs.50130;
  • GeneID 4692
  • KEGG hsa:4692;
  • Human Necdin Protein SEQUENCE
  • SEQUENCE 321 AA; 36086 MW; 5068FE121308BFAF CRC64;

    MSEQSKDLSD PNFAAEAPNS EVHSSPGVSE GVPPSATLAE PQSPPLGPTA

    APQAAPPPQA PNDEGDPKAL QQAAEEGRAH QAPSAAQPGP APPAPAQLVQ

    KAHELMWYVL VKDQKKMIIW FPDMVKDVIG SYKKWCRSIL RRTSLILARV

    FGLHLRLTSL HTMEFALVKA LEPEELDRVA LSNRMPMTGL LLMILSLIYV

    KGRGARESAV WNVLRILGLR PWKKHSTFGD VRKLITEEFV QMNYLKYQRV

    PYVEPPEYEF FWGSRASREI TKMQIMEFLA RVFKKDPQAW PSRYREALEE

    ARALREANPT AHYPRSSVSE D





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