Mouse Mup1 ELISA Kit

Alternative name
Mouse MUP 1 ELISA Kit;Mouse Gm2024 ELISA Kit;Mouse major urinary protein 12 ELISA Kit;
Catalog
E059378
species
Mouse
GeneMup1
Standard Curve
Other Species Human MUP1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Mup1 ELISA Kit allows for the in vitro quantitative determination of Mup1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalFor Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Intended Uses: This MUP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MUP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: MUP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MUP1 antibody and an MUP1-HRP conjugate. The assay sample and buffer are incubated together with MUP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUP1 concentration since MUP1 from samples and MUP1-HRP conjugate compete for the anti-MUP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MUP1 from the sample, fewer sites are left to bind MUP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUP1 concentration in each sample is interpolated from this standard curve.
Mouse Major urinary protein 1 Protein information
Uniprot ID MUP1_MOUSE
Uniprot AC P11588; Q61921;
UniGene Mm.335875; Mm.422695; Mm.457982; Mm.482072; Mm.482236;
GeneID 100039054
KEGG mmu:100039054;
Mouse Major urinary protein 1 Protein SEQUENCE
SEQUENCE 180 AA; 20648 MW; 5AA429BDA0700347 CRC64;
MKMLLLLCLG LTLVCVHAEE ASSTGRNFNV EKINGEWHTI ILASDKREKI
EDNGNFRLFL EQIHVLENSL VLKFHTVRDE ECSELSMVAD KTEKAGEYSV
TYDGFNTFTI PKTDYDNFLM AHLINEKDGE TFQLMGLYGR EPDLSSDIKE
RFAQLCEKHG ILRENIIDLS NANRCLQARE
UCSC uc008tay.3; mouse.;

Category

Mouse Mup1 ELISA Kit

Mouse MUP 1 ELISA Kit;Mouse Gm2024 ELISA Kit;Mouse major urinary protein 12 ELISA Kit;