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Human CD40 ELISA Kit

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  • Alternative name

    Human B-cell surface antigen CD40 ELISA Kit;Human Bp50 ELISA Kit;Human CD40L receptor ELISA Kit;Human CDW40 ELISA Kit;Human TNFRSF5 ELISA Kit;Human p50 ELISA Kit;Human CD40 molecule ELISA Kit;Human tumor necrosis factor receptor superfamily member 5 ELISA Kit;Human B cell surface antigen CD40 ELISA Kit;Human B cell-associated molecule ELISA Kit;Human CD40 molecule, TNF receptor superfamily member 5 ELISA Kit;
  • Catalog
    E007450
  • species
    Human
  • GeneCD40
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 u/mL
  • Intended UseHuman CD40 ELISA Kit allows for the in vitro quantitative determination of CD40 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This CPN IGG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPN IGG. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY CPN IGG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPN IGG antibody and an CPN IGG-HRP conjugate. The assay sample and buffer are incubated together with CPN IGG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPN IGG concentration since CPN IGG from samples and CPN IGG-HRP conjugate compete for the anti-CPN IGG antibody binding site. Since the number of sites is limited, a s more sites are occupied by CPN IGG from the sample, fewer sites are left to bind CPN IGG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPN IGG concentration in each sample is interpolated from this standard curve.
  • Human Tumor necrosis factor receptor superfamily member 5 Protein information
  • Uniprot ID TNR5_HUMAN
  • Uniprot AC P25942; E1P5S9; Q53GN5; Q5JY15; Q5U007; Q7M4Q8; Q86YK5; Q9BYU0;
  • UniGene Hs.472860;
  • GeneID 958
  • KEGG hsa:958;
  • Human Tumor necrosis factor receptor superfamily member 5 Protein SEQUENCE

  • SEQUENCE 277 AA; 30619 MW; BC8776EC2C4A5680 CRC64;

    MVRLPLQCVL WGCLLTAVHP EPPTACREKQ YLINSQCCSL CQPGQKLVSD

    CTEFTETECL PCGESEFLDT WNRETHCHQH KYCDPNLGLR VQQKGTSETD

    TICTCEEGWH CTSEACESCV LHRSCSPGFG VKQIATGVSD TICEPCPVGF

    FSNVSSAFEK CHPWTSCETK DLVVQQAGTN KTDVVCGPQD RLRALVVIPI

    IFGILFAILL VLVFIKKVAK KPTNKAPHPK QEPQEINFPD DLPGSNTAAP

    VQETLHGCQP VTQEDGKESR ISVQERQ

  • UCSC uc002xrg.2; human. [P25942-1];



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