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Human MMP14 ELISA Kit

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  • Alternative name

    Human MMP-14 ELISA Kit;Human MMP-X1 ELISA Kit;Human Membrane-type matrix metalloproteinase 1 ELISA Kit;Human MT-MMP 1 ELISA Kit;Human MTMMP1 ELISA Kit;Human membrane-type-1 matrix metalloproteinase ELISA Kit;Human MT1-MMP ELISA Kit;Human MT1MMP ELISA Kit;Human MT-MMP ELISA Kit;Human WNCHRS ELISA Kit;Human matrix metallopeptidase 14 ELISA Kit;Human matrix metalloproteinase-14 ELISA Kit;Human matrix metallopeptidase 14 (membrane-inserted) ELISA Kit;Human membrane type 1 metalloprotease ELISA Kit;
  • Catalog
    E023739
  • species
    Human
  • GeneMMP14
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human MMP-14. No significant cross-reactivity or interference between human MMP-14 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human MMP-14 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman MMP14 ELISA Kit allows for the in vitro quantitative determination of MMP14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Matrix metalloproteinase-14 Protein information
  • Uniprot ID MMP14_HUMAN
  • Uniprot AC P50281; A8K5L0; Q6GSF3; Q92678;
  • UniGene Hs.2399;
  • GeneID 4323
  • KEGG hsa:4323;
  • Human Matrix metalloproteinase-14 Protein SEQUENCE

  • SEQUENCE 582 AA; 65894 MW; 3722DE286A4BBCDE CRC64;

    MSPAPRPPRC LLLPLLTLGT ALASLGSAQS SSFSPEAWLQ QYGYLPPGDL

    RTHTQRSPQS LSAAIAAMQK FYGLQVTGKA DADTMKAMRR PRCGVPDKFG

    AEIKANVRRK RYAIQGLKWQ HNEITFCIQN YTPKVGEYAT YEAIRKAFRV

    WESATPLRFR EVPYAYIREG HEKQADIMIF FAEGFHGDST PFDGEGGFLA

    HAYFPGPNIG GDTHFDSAEP WTVRNEDLNG NDIFLVAVHE LGHALGLEHS

    SDPSAIMAPF YQWMDTENFV LPDDDRRGIQ QLYGGESGFP TKMPPQPRTT

    SRPSVPDKPK NPTYGPNICD GNFDTVAMLR GEMFVFKERW FWRVRNNQVM

    DGYPMPIGQF WRGLPASINT AYERKDGKFV FFKGDKHWVF DEASLEPGYP

    KHIKELGRGL PTDKIDAALF WMPNGKTYFF RGNKYYRFNE ELRAVDSEYP

    KNIKVWEGIP ESPRGSFMGS DEVFTYFYKG NKYWKFNNQK LKVEPGYPKS

    ALRDWMGCPS GGRPDEGTEE ETEVIIIEVD EEGGGAVSAA AVVLPVLLLL

    LVLAVGLAVF FFRRHGTPRR LLYCQRSLLD KV

  • UCSC uc001whc.5; human.;



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