Human ADAMTS5 ELISA Kit

Alternative name
Human ADAM-TS 5 ELISA Kit;Human ADAM-TS5 ELISA Kit;Human ADAMTS-5 ELISA Kit;Human a disintegrin and metalloproteinase with thrombospondin motifs 11 ELISA Kit;Human ADAM-TS 11 ELISA Kit;Human ADAMTS-11 ELISA Kit;Human ADMP-2 ELISA Kit;Human aggrecanase-2 ELISA Kit;Human ADAMTS11 ELISA Kit;Human ADMP2 ELISA Kit;Human ADAM metallopeptidase with thrombospondin type 1 motif 5 ELISA Kit;Human A disintegrin and metalloproteinase with thrombospondin motifs 5 ELISA Kit;Human a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) ELISA Kit;
Catalog
E000044
species
Human
GeneADAMTS5
Standard Curve
Other Species Mouse Adamts5 ELISA Kit Bovine ADAMTS5 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). No significant cross-reactivity or interference between A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.128ng/mL.
Intended UseHuman ADAMTS5 ELISA Kit allows for the in vitro quantitative determination of ADAMTS5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human A disintegrin and metalloproteinase with thrombospondin motifs 5 Protein information
Uniprot ID ATS5_HUMAN
Uniprot AC Q9UNA0; Q52LV4; Q9UKP2;
UniGene Hs.58324;
GeneID 11096
KEGG hsa:11096;
Human A disintegrin and metalloproteinase with thrombospondin motifs 5 Protein SEQUENCE
SEQUENCE 930 AA; 101718 MW; 8CCE448A15A29CE8 CRC64;
MLLGWASLLL CAFRLPLAAV GPAATPAQDK AGQPPTAAAA AQPRRRQGEE
VQERAEPPGH PHPLAQRRRS KGLVQNIDQL YSGGGKVGYL VYAGGRRFLL
DLERDGSVGI AGFVPAGGGT SAPWRHRSHC FYRGTVDGSP RSLAVFDLCG
GLDGFFAVKH ARYTLKPLLR GPWAEEEKGR VYGDGSARIL HVYTREGFSF
EALPPRASCE TPASTPEAHE HAPAHSNPSG RAALASQLLD QSALSPAGGS
GPQTWWRRRR RSISRARQVE LLLVADASMA RLYGRGLQHY LLTLASIANR
LYSHASIENH IRLAVVKVVV LGDKDKSLEV SKNAATTLKN FCKWQHQHNQ
LGDDHEEHYD AAILFTREDL CGHHSCDTLG MADVGTICSP ERSCAVIEDD
GLHAAFTVAH EIGHLLGLSH DDSKFCEETF GSTEDKRLMS SILTSIDASK
PWSKCTSATI TEFLDDGHGN CLLDLPRKQI LGPEELPGQT YDATQQCNLT
FGPEYSVCPG MDVCARLWCA VVRQGQMVCL TKKLPAVEGT PCGKGRICLQ
GKCVDKTKKK YYSTSSHGNW GSWGSWGQCS RSCGGGVQFA YRHCNNPAPR
NNGRYCTGKR AIYRSCSLMP CPPNGKSFRH EQCEAKNGYQ SDAKGVKTFV
EWVPKYAGVL PADVCKLTCR AKGTGYYVVF SPKVTDGTEC RLYSNSVCVR
GKCVRTGCDG IIGSKLQYDK CGVCGGDNSS CTKIVGTFNK KSKGYTDVVR
IPEGATHIKV RQFKAKDQTR FTAYLALKKK NGEYLINGKY MISTSETIID
INGTVMNYSG WSHRDDFLHG MGYSATKEIL IVQILATDPT KPLDVRYSFF
VPKKSTPKVN SVTSHGSNKV GSHTSQPQWV TGPWLACSRT CDTGWHTRTV
QCQDGNRKLA KGCPLSQRPS AFKQCLLKKC
UCSC uc002ymg.4; human.;

Category

Human ADAMTS5 ELISA Kit