Human FGF9 ELISA Kit

Alternative name
Human FGF-9 ELISA Kit;Human Glia-activating factor ELISA Kit;Human GAF ELISA Kit;Human heparin-binding growth factor 9 ELISA Kit;Human HBGF-9 ELISA Kit;Human HBFG-9 ELISA Kit;Human SYNS3 ELISA Kit;Human fibroblast growth factor 9 ELISA Kit;Human fibroblast growth factor 9 (glia-activating factor) ELISA Kit;
Catalog
E014780
species
Human
GeneFGF9
SpecificityNatural and recombinant human FGF9
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 15pg/ml
Intended UseHuman FGF9 ELISA Kit allows for the in vitro quantitative determination of FGF9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human FGF9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF9 has been precoated onto 96-well plates. Standards(sf21,M1-S208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF9 amount of sample captured in plate. Background: Fibroblast growth factor-9 (FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells.1 The human FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208amino acids. Sequence similarity to other members of the FGF family has been estimated to be around 30%.2FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma.3 FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for bothprostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia.4 As a result of glycosylation, the molecular mass is 25-27KDa.
Human Fibroblast growth factor 9 Protein information
Uniprot ID FGF9_HUMAN
Uniprot AC P31371; A8K427; Q3SY32;
UniGene Hs.111;
GeneID 2254
KEGG hsa:2254;
Human Fibroblast growth factor 9 Protein SEQUENCE
SEQUENCE 208 AA; 23441 MW; F32A0E7106EF59C9 CRC64;
MAPLGEVGNY FGVQDAVPFG NVPVLPVDSP VLLSDHLGQS EAGGLPRGPA
VTDLDHLKGI LRRRQLYCRT GFHLEIFPNG TIQGTRKDHS RFGILEFISI
AVGLVSIRGV DSGLYLGMNE KGELYGSEKL TQECVFREQF EENWYNTYSS
NLYKHVDTGR RYYVALNKDG TPREGTRTKR HQKFTHFLPR PVDPDKVPEL
YKDILSQS
UCSC uc001uog.3; human.;

Human FGF9 ELISA Kit

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