Human LIPG ELISA Kit

Alternative name
Human endothelial cell-derived lipase ELISA Kit;Human EDL ELISA Kit;Human EL ELISA Kit;Human PRO719 ELISA Kit;Human lipase G, endothelial type ELISA Kit;Human endothelial lipase ELISA Kit;Human lipase, endothelial ELISA Kit;Human lipoprotein lipase H ELISA Kit;
Catalog
E022777
species
Human
GeneLIPG
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human LIPG. No significant cross-reactivity or interference between Human LIPG and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.156 ng/ml
Intended UseHuman LIPG ELISA Kit allows for the in vitro quantitative determination of LIPG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Endothelial lipase (EL) is a newly described member of the triglyceride lipase gene family. It has a considerable molecular homology with lipoprotein lipase (LPL) (44%) and hepatic lipase (HL) (41%). Unlike LPL and HL, this enzyme is synthesized by endothelial cells and functions at the site where it is synthesized. Furthermore, its tissue distribution is different from that of LPL and HL. Unlike LPL and HL, EL is located in the vascular endothelial cells and its expression is highly regulated by cytokines and physical forces, suggesting that it may play a role in the development of atherosclerosis. As a lipase, EL has primarily phospholipase A1 activity. Animals that overexpress EL showed reduced HDL cholesterol levels. Conversely, animals that are deficient in EL showed a marked elevation in HDL cholesterol levels, suggesting that it is a major genetic determinant for the concentration, structure, and metabolism of HDL, which protects against atherosclerosis. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for EL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain EL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of EL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Endothelial lipase Protein information
Uniprot ID LIPE_HUMAN
Uniprot AC Q9Y5X9; B0LPG6; Q6P9C8; Q6UW82;
UniGene Hs.465102;
GeneID 9388
KEGG hsa:9388;
Human Endothelial lipase Protein SEQUENCE
SEQUENCE 500 AA; 56795 MW; 96EC95125CD2A3FD CRC64;
MSNSVPLLCF WSLCYCFAAG SPVPFGPEGR LEDKLHKPKA TQTEVKPSVR
FNLRTSKDPE HEGCYLSVGH SQPLEDCSFN MTAKTFFIIH GWTMSGIFEN
WLHKLVSALH TREKDANVVV VDWLPLAHQL YTDAVNNTRV VGHSIARMLD
WLQEKDDFSL GNVHLIGYSL GAHVAGYAGN FVKGTVGRIT GLDPAGPMFE
GADIHKRLSP DDADFVDVLH TYTRSFGLSI GIQMPVGHID IYPNGGDFQP
GCGLNDVLGS IAYGTITEVV KCEHERAVHL FVDSLVNQDK PSFAFQCTDS
NRFKKGICLS CRKNRCNSIG YNAKKMRNKR NSKMYLKTRA GMPFRVYHYQ
MKIHVFSYKN MGEIEPTFYV TLYGTNADSQ TLPLEIVERI EQNATNTFLV
YTEEDLGDLL KIQLTWEGAS QSWYNLWKEF RSYLSQPRNP GRELNIRRIR
VKSGETQRKL TFCTEDPENT SISPGRELWF RKCRDGWRMK NETSPTVELP
UCSC uc002ldu.2; human. [Q9Y5X9-1];

Category

Human LIPG ELISA Kit

Human endothelial cell-derived lipase ELISA Kit;Human EDL ELISA Kit;Human EL ELISA Kit;Human PRO719 ELISA Kit;Human lipase G, endothelial type ELISA Kit;Human endothelial lipase ELISA Kit;Human lipase, endothelial ELISA Kit;Human lipoprotein lipase H ELISA Kit;