Human SMAD1 ELISA Kit

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  • Alternative name

    Human MAD homolog 1 ELISA Kit;Human mothers against DPP homolog 1 ELISA Kit;Human JV4-1 ELISA Kit;Human Mad-related protein 1 ELISA Kit;Human SMAD family member 1 ELISA Kit;Human SMAD 1 ELISA Kit;Human Smad1 ELISA Kit;Human hSMAD1 ELISA Kit;Human Transforming growth factor-beta-signaling protein 1 ELISA Kit;Human BSP-1 ELISA Kit;Human BSP1 ELISA Kit;Human MADH1 ELISA Kit;Human MADR1 ELISA Kit;Human JV41 ELISA Kit;Human mothers against decapentaplegic homolog 1 ELISA Kit;Human MAD, mothers against decapentaplegic homolog 1 ELISA Kit;Human SMAD, mothers against DPP homolog 1 ELISA Kit;Human TGF-beta signaling protein 1 ELISA Kit;Human transforming growth factor-beta signaling protein 1 ELISA Kit;

  • Catalog
    E024914
  • species
    Human
  • GeneSMAD1
  • Standard CurveHuman SMAD1 ELISA Kit
  • Other Species Human Smad1/MADH1 ELISA KitMouse Smad1 ELISA KitBovine SMAD1 ELISA KitRat Smad1 ELISA Kit
  • SpecificityThis assay has high sensitivity for detection of Smad1. 100% cross-reactivity of Smad1 was observed among human, mouse, rat and bovine.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Smad1 is typically less than 0.132ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
  • Intended UseHuman SMAD1 ELISA Kit allows for the in vitro quantitative determination of SMAD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Smad1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Smad1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Smad1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Smad1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Mothers against decapentaplegic homolog 1 Protein information
  • Uniprot ID SMAD1_HUMAN
  • Uniprot AC Q15797; A8KAJ0; D3DNZ9; Q16636; Q9UFT8;
  • UniGene Hs.604588;
  • GeneID 4086
  • KEGG hsa:4086;
  • Human Mothers against decapentaplegic homolog 1 Protein SEQUENCE
  • SEQUENCE 465 AA; 52260 MW; 2DD34B7F434DBC7E CRC64;

    MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE

    LEKALSCPGQ PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH

    HELKPLECCE FPFGSKQKEV CINPYHYKRV ESPVLPPVLV PRHSEYNPQH

    SLLAQFRNLG QNEPHMPLNA TFPDSFQQPN SHPFPHSPNS SYPNSPGSSS

    STYPHSPTSS DPGSPFQMPA DTPPPAYLPP EDPMTQDGSQ PMDTNMMAPP

    LPSEINRGDV QAVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT

    DPSNNKNRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD

    SSIFVQSRNC NYHHGFHPTT VCKIPSGCSL KIFNNQEFAQ LLAQSVNHGF

    ETVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV

    LTQMGSPHNP ISSVS

  • UCSC uc003ikc.4; human. [Q15797-1];


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