Mouse Mecp2 ELISA Kit

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  • Alternative name

    Mouse meCp-2 protein ELISA Kit;Mouse MeCp2 ELISA Kit;Mouse 1500041B07Rik ELISA Kit;Mouse D630021H01Rik ELISA Kit;Mouse Mbd5 ELISA Kit;Mouse WBP10 ELISA Kit;Mouse methyl CpG binding protein 2 ELISA Kit;Mouse methyl-CpG-binding protein 2 ELISA Kit;

  • Catalog
    E060015
  • species
    Mouse
  • GeneMecp2
  • Standard CurveMouse Mecp2 ELISA Kit
  • Other Species Human MECP2 ELISA KitMonkey MECP2 ELISA KitRat Mecp2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Methyl CpG Binding Protein 2 (MECP2). No significant cross-reactivity or interference between Methyl CpG Binding Protein 2 (MECP2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.102ng/mL.
  • Intended UseMouse Mecp2 ELISA Kit allows for the in vitro quantitative determination of Mecp2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methyl CpG Binding Protein 2 (MECP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methyl CpG Binding Protein 2 (MECP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methyl CpG Binding Protein 2 (MECP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methyl CpG Binding Protein 2 (MECP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Methyl-CpG-binding protein 2 Protein information
  • Uniprot ID MECP2_MOUSE
  • Uniprot AC Q9Z2D6; B1AUZ2; B1AUZ3; Q3TYG1;
  • UniGene Mm.131408; Mm.414303;
  • GeneID 17257
  • KEGG mmu:17257;
  • Mouse Methyl-CpG-binding protein 2 Protein SEQUENCE
  • SEQUENCE 484 AA; 52307 MW; 62FD228F0118A49F CRC64;

    MVAGMLGLRE EKSEDQDLQG LRDKPLKFKK AKKDKKEDKE GKHEPLQPSA

    HHSAEPAEAG KAETSESSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL

    PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL

    DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK APGTGRGRGR PKGSGTGRPK

    AAASEGVQVK RVLEKSPGKL VVKMPFQASP GGKGEGGGAT TSAQVMVIKR

    PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVHETV

    LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS

    PKGRSSSASS PPKKEHHHHH HHSESTKAPM PLLPSPPPPE PESSEDPISP

    PEPQDLSSSI CKEEKMPRGG SLESDGCPKE PAKTQPMVAT TTTVAEKYKH

    RGEGERKDIV SSSMPRPNRE EPVDSRTPVT ERVS

  • UCSC uc009tnu.3; mouse. [Q9Z2D6-2];


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