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Catalog
E022378
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species
Human
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GeneLeptospira
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman Leptospira ELISA Kit allows for the in vitro quantitative determination of Leptospira , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells and any antibodies specific for the antigen present will bind to the pre-coated antigen. After removal of unbound material, alkaline phosphatase-conjugated anti-human IgG is added to each well and react with the immune complex. Finally, substrate solution rho-nitrophenylphosphate is added to each well. The enzyme-substrate reaction is terminated by the addition of sodium hydroxide and the color change is measured spectrophotometrically at a wavelength of 405 nm +/- 2 nm. Calculate the concentration of human leptospira IgG in the samples.
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