Human COCH ELISA Kit

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  • Alternative name

    Human COCH-5B2 ELISA Kit;Human COCH5B2 ELISA Kit;Human DFNA9 ELISA Kit;Human cochlin ELISA Kit;Human coagulation factor C homolog, cochlin (Limulus polyphemus) ELISA Kit;

  • Catalog
    E009905
  • species
    Human
  • GeneCOCH
  • Standard CurveHuman COCH ELISA Kit
  • Other Species Mouse Coch ELISA KitMouse COCH-IgG ELISA KitBovine COCH ELISA KitGuinea Pig COCH ELISA KitChicken COCH ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of COCH. No significant cross-reactivity or interference between COCH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COCH and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman COCH ELISA Kit allows for the in vitro quantitative determination of COCH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the assay: COCH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COCH antibody and an COCH-HRP conjugate. The assay sample and buffer are incubated together with COCH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COCH concentration since COCH from samples and COCH-HRP conjugate compete for the anti-COCH antibody binding site. Since the number of sites is limited, as more sites are occupied by COCH from the sample, fewer sites are left to bind COCH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COCH concentration in each sample is interpolated from this standard curve.
  • Human Cochlin Protein information
  • Uniprot ID COCH_HUMAN
  • Uniprot AC O43405; A8K9K9; D3DS84; Q96IU6;
  • UniGene Hs.21016;
  • GeneID 1690
  • KEGG hsa:1690;
  • Human Cochlin Protein SEQUENCE
  • SEQUENCE 550 AA; 59483 MW; 74D7D51290098B30 CRC64;

    MSAAWIPALG LGVCLLLLPG PAGSEGAAPI AITCFTRGLD IRKEKADVLC

    PGGCPLEEFS VYGNIVYASV SSICGAAVHR GVISNSGGPV RVYSLPGREN

    YSSVDANGIQ SQMLSRWSAS FTVTKGKSST QEATGQAVST AHPPTGKRLK

    KTPEKKTGNK DCKADIAFLI DGSFNIGQRR FNLQKNFVGK VALMLGIGTE

    GPHVGLVQAS EHPKIEFYLK NFTSAKDVLF AIKEVGFRGG NSNTGKALKH

    TAQKFFTVDA GVRKGIPKVV VVFIDGWPSD DIEEAGIVAR EFGVNVFIVS

    VAKPIPEELG MVQDVTFVDK AVCRNNGFFS YHMPNWFGTT KYVKPLVQKL

    CTHEQMMCSK TCYNSVNIAF LIDGSSSVGD SNFRLMLEFV SNIAKTFEIS

    DIGAKIAAVQ FTYDQRTEFS FTDYSTKENV LAVIRNIRYM SGGTATGDAI

    SFTVRNVFGP IRESPNKNFL VIVTDGQSYD DVQGPAAAAH DAGITIFSVG

    VAWAPLDDLK DMASKPKESH AFFTREFTGL EPIVSDVIRG ICRDFLESQQ

  • UCSC uc001wqp.3; human. [O43405-1];


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