Human COCH ELISA Kit

Alternative name
Human COCH-5B2 ELISA Kit;Human COCH5B2 ELISA Kit;Human DFNA9 ELISA Kit;Human cochlin ELISA Kit;Human coagulation factor C homolog, cochlin (Limulus polyphemus) ELISA Kit;
Catalog
E009905
species
Human
GeneCOCH
SpecificityThis assay has high sensitivity and excellent specificity for detection of COCH. No significant cross-reactivity or interference between COCH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COCH and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman COCH ELISA Kit allows for the in vitro quantitative determination of COCH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: COCH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COCH antibody and an COCH-HRP conjugate. The assay sample and buffer are incubated together with COCH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COCH concentration since COCH from samples and COCH-HRP conjugate compete for the anti-COCH antibody binding site. Since the number of sites is limited, as more sites are occupied by COCH from the sample, fewer sites are left to bind COCH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COCH concentration in each sample is interpolated from this standard curve.
Human Cochlin Protein information
Uniprot ID COCH_HUMAN
Uniprot AC O43405; A8K9K9; D3DS84; Q96IU6;
UniGene Hs.21016;
GeneID 1690
KEGG hsa:1690;
Human Cochlin Protein SEQUENCE
SEQUENCE 550 AA; 59483 MW; 74D7D51290098B30 CRC64;
MSAAWIPALG LGVCLLLLPG PAGSEGAAPI AITCFTRGLD IRKEKADVLC
PGGCPLEEFS VYGNIVYASV SSICGAAVHR GVISNSGGPV RVYSLPGREN
YSSVDANGIQ SQMLSRWSAS FTVTKGKSST QEATGQAVST AHPPTGKRLK
KTPEKKTGNK DCKADIAFLI DGSFNIGQRR FNLQKNFVGK VALMLGIGTE
GPHVGLVQAS EHPKIEFYLK NFTSAKDVLF AIKEVGFRGG NSNTGKALKH
TAQKFFTVDA GVRKGIPKVV VVFIDGWPSD DIEEAGIVAR EFGVNVFIVS
VAKPIPEELG MVQDVTFVDK AVCRNNGFFS YHMPNWFGTT KYVKPLVQKL
CTHEQMMCSK TCYNSVNIAF LIDGSSSVGD SNFRLMLEFV SNIAKTFEIS
DIGAKIAAVQ FTYDQRTEFS FTDYSTKENV LAVIRNIRYM SGGTATGDAI
SFTVRNVFGP IRESPNKNFL VIVTDGQSYD DVQGPAAAAH DAGITIFSVG
VAWAPLDDLK DMASKPKESH AFFTREFTGL EPIVSDVIRG ICRDFLESQQ
UCSC uc001wqp.3; human. [O43405-1];

Category

Human COCH ELISA Kit

Human COCH-5B2 ELISA Kit;Human COCH5B2 ELISA Kit;Human DFNA9 ELISA Kit;Human cochlin ELISA Kit;Human coagulation factor C homolog, cochlin (Limulus polyphemus) ELISA Kit;