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Alternative name
Laminin subunit gamma-2 ELISA KIT; Cell-scattering factor 140 kDa subunit ELISA KIT; CSF 140 kDa subunit ELISA KIT; Epiligrin subunit gamma ELISA KIT; Kalinin subunit gamma ELISA KIT; Kalinin/nicein/epiligrin 100 kDa subunit ELISA KIT; Ladsin 140 kDa subunit ELISA KIT; Laminin B2t chain ELISA KIT; Laminin-5 subunit gamma ELISA KIT; Large adhesive scatter factor 140 kDa subunit ELISA KIT; Nicein subunit gammaLAMC2 ELISA KIT; LAMB2T ELISA KIT; LAMNB2 ELISA KIT; CSF 140 kDa subunit ELISA KIT
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Catalog
E022146
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species
Human
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GeneLN
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Laminin (LN).
No significant cross-reactivity or interference between Laminin (LN) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 13.6pg/mL.
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Intended UseHuman LN ELISA Kit allows for the in vitro quantitative determination of LN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin (LN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin (LN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin (LN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin (LN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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