Human IgG ELISA Kit

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  • Catalog
    E019583
  • species
    Human
  • GeneIgG
  • Standard CurveHuman IgG ELISA Kit
  • Other Species Human ACA-IgG ELISA KitHuman AAV-IgG ELISA KitHuman A-FODRIN IgG/IgA ELISA KitHuman APA IgG ELISA KitHuman A Fodrin IgG/IgA ELISA KitHuman ACA IgG ELISA KitHuman EMA (IgG) ELISA KitHuman AnuA IgG ELISA KitHuman PR3 ab IgG ELISA KitHuman ACA (IgG) ELISA KitHuman EMA IgG ELISA KitHuman EHF IgG ELISA KitHuman Hp-IgG ELISA KitHuman HBcAb-IgG ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG). No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.46ug/mL.
  • Intended UseHuman IgG ELISA Kit allows for the in vitro quantitative determination of IgG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin G (IgG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample.



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