Human MMP25 ELISA Kit

Alternative name
Human MMP-25 ELISA Kit;Human leukolysin ELISA Kit;Human membrane-type matrix metalloproteinase 6 ELISA Kit;Human MT-MMP 6 ELISA Kit;Human MTMMP6 ELISA Kit;Human Membrane-type-6 matrix metalloproteinase ELISA Kit;Human MT6-MMP ELISA Kit;Human MT6MMP ELISA Kit;Human MMP20 ELISA Kit;Human MMPL1 ELISA Kit;Human MMP20A ELISA Kit;Human MT-MMP6 ELISA Kit;Human matrix metallopeptidase 25 ELISA Kit;Human matrix metalloproteinase-25 ELISA Kit;Human matrix metallopeptidase-like 1 ELISA Kit;Human matrix metalloproteinase 20 ELISA Kit;Human matrix metalloproteinase-like 1 ELISA Kit;Human membrane-type 6 matrix metalloproteinase ELISA Kit;
Catalog
E023793
species
Human
GeneMMP25
SpecificityThis assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 25 (MMP25). No significant cross-reactivity or interference between Matrix Metalloproteinase 25 (MMP25) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.065ng/mL.
Intended UseHuman MMP25 ELISA Kit allows for the in vitro quantitative determination of MMP25 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 25 (MMP25). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 25 (MMP25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 25 (MMP25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 25 (MMP25) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Matrix metalloproteinase-25 Protein information
Uniprot ID MMP25_HUMAN
Uniprot AC Q9NPA2; D3DUA8; Q9H3Q0;
UniGene Hs.654979;
GeneID 64386
KEGG hsa:64386;
Human Matrix metalloproteinase-25 Protein SEQUENCE
SEQUENCE 562 AA; 62554 MW; A6A50AE05D969C64 CRC64;
MRLRLRLLAL LLLLLAPPAR APKPSAQDVS LGVDWLTRYG YLPPPHPAQA
QLQSPEKLRD AIKVMQRFAG LPETGRMDPG TVATMRKPRC SLPDVLGVAG
LVRRRRRYAL SGSVWKKRTL TWRVRSFPQS SQLSQETVRV LMSYALMAWG
MESGLTFHEV DSPQGQEPDI LIDFARAFHQ DSYPFDGLGG TLAHAFFPGE
HPISGDTHFD DEETWTFGSK DGEGTDLFAV AVHEFGHALG LGHSSAPNSI
MRPFYQGPVG DPDKYRLSQD DRDGLQQLYG KAPQTPYDKP TRKPLAPPPQ
PPASPTHSPS FPIPDRCEGN FDAIANIRGE TFFFKGPWFW RLQPSGQLVS
PRPARLHRFW EGLPAQVRVV QAAYARHRDG RILLFSGPQF WVFQDRQLEG
GARPLTELGL PPGEEVDAVF SWPQNGKTYL VRGRQYWRYD EAAARPDPGY
PRDLSLWEGA PPSPDDVTVS NAGDTYFFKG AHYWRFPKNS IKTEPDAPQP
MGPNWLDCPA PSSGPRAPRP PKATPVSETC DCQCELNQAA GRWPAPIPLL
LLPLLVGGVA SR
UCSC uc002cth.4; human.;

Category

Human MMP25 ELISA Kit