Human KLB ELISA Kit

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  • Alternative name

    Human BKL ELISA Kit;Human betaKlotho ELISA Kit;Human klotho beta-like protein ELISA Kit;Human klotho beta ELISA Kit;Human beta-klotho ELISA Kit;Human b-Klotho ELISA Kit;Human klotho beta like ELISA Kit;

  • Catalog
    E021916
  • species
    Human
  • GeneKLB
  • Other Species Mouse Klb ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human KLB. No significant cross-reactivity or interference between Human KLB and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity15.6 pg/ml
  • Intended UseHuman KLB ELISA Kit allows for the in vitro quantitative determination of KLB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLB bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Beta-klotho Protein information
  • Uniprot ID KLOTB_HUMAN
  • Uniprot AC Q86Z14; Q2M3K8;
  • UniGene Hs.90756;
  • GeneID 152831
  • KEGG hsa:152831;
  • Human Beta-klotho Protein SEQUENCE
  • SEQUENCE 1044 AA; 119808 MW; 6EA0198250F8928F CRC64;

    MKPGCAAGSP GNEWIFFSTD EITTRYRNTM SNGGLQRSVI LSALILLRAV

    TGFSGDGRAI WSKNPNFTPV NESQLFLYDT FPKNFFWGIG TGALQVEGSW

    KKDGKGPSIW DHFIHTHLKN VSSTNGSSDS YIFLEKDLSA LDFIGVSFYQ

    FSISWPRLFP DGIVTVANAK GLQYYSTLLD ALVLRNIEPI VTLYHWDLPL

    ALQEKYGGWK NDTIIDIFND YATYCFQMFG DRVKYWITIH NPYLVAWHGY

    GTGMHAPGEK GNLAAVYTVG HNLIKAHSKV WHNYNTHFRP HQKGWLSITL

    GSHWIEPNRS ENTMDIFKCQ QSMVSVLGWF ANPIHGDGDY PEGMRKKLFS

    VLPIFSEAEK HEMRGTADFF AFSFGPNNFK PLNTMAKMGQ NVSLNLREAL

    NWIKLEYNNP RILIAENGWF TDSRVKTEDT TAIYMMKNFL SQVLQAIRLD

    EIRVFGYTAW SLLDGFEWQD AYTIRRGLFY VDFNSKQKER KPKSSAHYYK

    QIIRENGFSL KESTPDVQGQ FPCDFSWGVT ESVLKPESVA SSPQFSDPHL

    YVWNATGNRL LHRVEGVRLK TRPAQCTDFV NIKKQLEMLA RMKVTHYRFA

    LDWASVLPTG NLSAVNRQAL RYYRCVVSEG LKLGISAMVT LYYPTHAHLG

    LPEPLLHADG WLNPSTAEAF QAYAGLCFQE LGDLVKLWIT INEPNRLSDI

    YNRSGNDTYG AAHNLLVAHA LAWRLYDRQF RPSQRGAVSL SLHADWAEPA

    NPYADSHWRA AERFLQFEIA WFAEPLFKTG DYPAAMREYI ASKHRRGLSS

    SALPRLTEAE RRLLKGTVDF CALNHFTTRF VMHEQLAGSR YDSDRDIQFL

    QDITRLSSPT RLAVIPWGVR KLLRWVRRNY GDMDIYITAS GIDDQALEDD

    RLRKYYLGKY LQEVLKAYLI DKVRIKGYYA FKLAEEKSKP RFGFFTSDFK

    AKSSIQFYNK VISSRGFPFE NSSSRCSQTQ ENTECTVCLF LVQKKPLIFL

    GCCFFSTLVL LLSIAIFQRQ KRRKFWKAKN LQHIPLKKGK RVVS

  • UCSC uc003gua.4; human.;



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