Human alpha1-AT ELISA Kit

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  • Alternative name

    Alpha-1-antitrypsin ELISA KIT; Alpha-1 protease inhibitor ELISA KIT; Alpha-1-antiproteinase ELISA KIT; Serpin A1SERPINA1 ELISA KIT; AAT ELISA KIT; PI ELISA KIT; SPAAT ELISA KIT

  • Catalog
  • species
  • Genealpha1-AT
  • Other Species Mouse alpha1-AT ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<1.875ng/ml
  • Intended UseHuman alpha1-AT ELISA Kit allows for the in vitro quantitative determination of alpha1-AT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human alpha1-AT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human alpha1-AT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human alpha1-AT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human alpha1-AT can be calculated.

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