Human IL5RA ELISA Kit

Alternative name
Human IL-5 receptor subunit alpha ELISA Kit;Human IL-5R subunit alpha ELISA Kit;Human IL-5R-alpha ELISA Kit;Human IL-5RA ELISA Kit;Human CDw125 ELISA Kit;Human IL5R ELISA Kit;Human CD125 ELISA Kit;Human HSIL5R3 ELISA Kit;Human interleukin 5 receptor subunit alpha ELISA Kit;Human interleukin-5 receptor subunit alpha ELISA Kit;Human CD125 antigen ELISA Kit;Human interleukin 5 receptor type 3 ELISA Kit;Human interleukin 5 receptor, alpha ELISA Kit;Human interleukin-5 receptor alpha chain ELISA Kit;
Catalog
E020763
species
Human
GeneIL5RA
SpecificityThis assay has high sensitivity and excellent specificity for detection of IL5Ra. No significant cross-reactivity or interference between IL5Ra and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human IL5Ra is typically less than24.6pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
Intended UseHuman IL5RA ELISA Kit allows for the in vitro quantitative determination of IL5RA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL5Ra. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IL5Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL5Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL5Ra in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Interleukin-5 receptor subunit alpha Protein information
Uniprot ID IL5RA_HUMAN
Uniprot AC Q01344; B3IU77; B4E2G0; Q14633; Q15469; Q6ISX9;
UniGene Hs.68876;
GeneID 3568
KEGG hsa:3568;
Human Interleukin-5 receptor subunit alpha Protein SEQUENCE
SEQUENCE 420 AA; 47685 MW; 4E0A5F2838B9C4FE CRC64;
MIIVAHVLLI LLGATEILQA DLLPDEKISL LPPVNFTIKV TGLAQVLLQW
KPNPDQEQRN VNLEYQVKIN APKEDDYETR ITESKCVTIL HKGFSASVRT
ILQNDHSLLA SSWASAELHA PPGSPGTSIV NLTCTTNTTE DNYSRLRSYQ
VSLHCTWLVG TDAPEDTQYF LYYRYGSWTE ECQEYSKDTL GRNIACWFPR
TFILSKGRDW LAVLVNGSSK HSAIRPFDQL FALHAIDQIN PPLNVTAEIE
GTRLSIQWEK PVSAFPIHCF DYEVKIHNTR NGYLQIEKLM TNAFISIIDD
LSKYDVQVRA AVSSMCREAG LWSEWSQPIY VGNDEHKPLR EWFVIVIMAT
ICFILLILSL ICKICHLWIK LFPPIPAPKS NIKDLFVTTN YEKAGSSETE
IEVICYIEKP GVETLEDSVF
UCSC uc010hbs.4; human. [Q01344-1];

Category

Human IL5RA ELISA Kit