Mouse Alpl ELISA Kit

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  • Alternative name

    Mouse AP-TNAP ELISA Kit;Mouse TNSALP ELISA Kit;Mouse Alkaline phosphatase 2 ELISA Kit;Mouse Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit;Mouse Akp-2 ELISA Kit;Mouse Akp2 ELISA Kit;Mouse ALP ELISA Kit;Mouse APTNAP ELISA Kit;Mouse TNAP ELISA Kit;Mouse alkaline phosphatase, liver/bone/kidney ELISA Kit;Mouse alkaline phosphatase, tissue-nonspecific isozyme ELISA Kit;Mouse alkaline phosphatase 2, liver ELISA Kit;

  • Catalog
    E043237
  • species
    Mouse
  • GeneAlpl
  • Standard CurveMouse Alpl ELISA Kit
  • Other Species Human ALPL ELISA KitBovine ALPL ELISA KitChicken ALPL ELISA KitRat Alpl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). No significant cross-reactivity or interference between Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.062ng/mL.
  • Intended UseMouse Alpl ELISA Kit allows for the in vitro quantitative determination of Alpl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Liver/Bone/Kidney (ALPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Alkaline phosphatase, tissue-nonspecific isozyme Protein information
  • Uniprot ID PPBT_MOUSE
  • Uniprot AC P09242; Q6P1B0;
  • UniGene Mm.288186;
  • GeneID 11647
  • KEGG mmu:11647;
  • Mouse Alkaline phosphatase, tissue-nonspecific isozyme Protein SEQUENCE
  • SEQUENCE 524 AA; 57514 MW; 59D99110C60FA050 CRC64;

    MISPFLVLAI GTCLTNSFVP EKERDPSYWR QQAQETLKNA LKLQKLNTNV

    AKNVIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT

    YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI

    LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG

    CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL

    DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN

    RNNLTDPSLS EMVEVALRIL TKNLKGFFLL VEGGRIDHGH HEGKAKQALH

    EAVEMDQAIG KAGAMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP

    MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR

    HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA

    GSGSAPSPGA LLLPLAVLSL RTLF

  • UCSC uc008vjr.3; mouse.;


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