Human F2 ELISA Kit

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  • Alternative name

    Human Coagulation factor II ELISA Kit;Human PT ELISA Kit;Human RPRGL2 ELISA Kit;Human THPH1 ELISA Kit;Human coagulation factor II, thrombin ELISA Kit;Human prothrombin ELISA Kit;Human prepro-coagulation factor II ELISA Kit;Human prothrombin B-chain ELISA Kit;Human serine protease ELISA Kit;

  • Catalog
    E009806
  • species
    Human
  • GeneF2
  • Standard CurveHuman F2 ELISA Kit
  • Other Species Mouse F2 ELISA KitBovine F2 ELISA KitPorcine F2 ELISA KitRat F2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Coagulation Factor II (F2). No significant cross-reactivity or interference between Coagulation Factor II (F2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.17ng/mL.
  • Intended UseHuman F2 ELISA Kit allows for the in vitro quantitative determination of F2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor II (F2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor II (F2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor II (F2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor II (F2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Thrombin heavy chain Protein information
  • Uniprot ID THRB_HUMAN
  • Uniprot AC Q9UCA1;
  • UniGene Hs.655207;
  • GeneID 2147
  • KEGG hsa:2147;
  • Human Thrombin heavy chain Protein SEQUENCE
  • SEQUENCE 622 AA; 70037 MW; 8A25E1DA88208FCF CRC64;

    MAHVRGLQLP GCLALAALCS LVHSQHVFLA PQQARSLLQR VRRANTFLEE

    VRKGNLEREC VEETCSYEEA FEALESSTAT DVFWAKYTAC ETARTPRDKL

    AACLEGNCAE GLGTNYRGHV NITRSGIECQ LWRSRYPHKP EINSTTHPGA

    DLQENFCRNP DSSTTGPWCY TTDPTVRRQE CSIPVCGQDQ VTVAMTPRSE

    GSSVNLSPPL EQCVPDRGQQ YQGRLAVTTH GLPCLAWASA QAKALSKHQD

    FNSAVQLVEN FCRNPDGDEE GVWCYVAGKP GDFGYCDLNY CEEAVEEETG

    DGLDEDSDRA IEGRTATSEY QTFFNPRTFG SGEADCGLRP LFEKKSLEDK

    TERELLESYI DGRIVEGSDA EIGMSPWQVM LFRKSPQELL CGASLISDRW

    VLTAAHCLLY PPWDKNFTEN DLLVRIGKHS RTRYERNIEK ISMLEKIYIH

    PRYNWRENLD RDIALMKLKK PVAFSDYIHP VCLPDRETAA SLLQAGYKGR

    VTGWGNLKET WTANVGKGQP SVLQVVNLPI VERPVCKDST RIRITDNMFC

    AGYKPDEGKR GDACEGDSGG PFVMKSPFNN RWYQMGIVSW GEGCDRDGKY

    GFYTHVFRLK KWIQKVIDQF GE

  • UCSC uc001ndf.5; human.;



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