Human AMOT ELISA Kit

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  • Alternative name

    Human KIAA1071 ELISA Kit;Human angiomotin ELISA Kit;Human angiomotin p130 isoform ELISA Kit;Human angiomotin p80 isoform ELISA Kit;

  • Catalog
    E002026
  • species
    Human
  • GeneAMOT
  • Standard CurveHuman AMOT ELISA Kit
  • Other Species Mouse Amot ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AMOT. No significant cross-reactivity or interference between AMOT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AMOT and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman AMOT ELISA Kit allows for the in vitro quantitative determination of AMOT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: AMOT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMOT antibody and an AMOT-HRP conjugate. The assay sample and buffer are incubated together with AMOT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMOT concentration since AMOT from samples and AMOT-HRP conjugate compete for the anti-AMOT antibody binding site. Since the number of sites is limited, as more sites are occupied by AMOT from the sample, fewer sites are left to bind AMOT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMOT concentration in each sample is interpolated from this standard curve.
  • Human Angiomotin Protein information
  • Uniprot ID AMOT_HUMAN
  • Uniprot AC Q4VCS5; Q504X5; Q9HD27; Q9UPT1;
  • UniGene Hs.528051;
  • GeneID 154796
  • KEGG hsa:154796;
  • Human Angiomotin Protein SEQUENCE
  • SEQUENCE 1084 AA; 118085 MW; D7E7021E9535A628 CRC64;

    MRNSEEQPSG GTTVLQRLLQ EQLRYGNPSE NRSLLAIHQQ ATGNGPPFPS

    GSGNPGPQSD VLSPQDHHQQ LVAHAARQEP QGQEIQSENL IMEKQLSPRM

    QNNEELPTYE EAKVQSQYFR GQQHASVGAA FYVTGVTNQK MRTEGRPSVQ

    RLNPGKMHQD EGLRDLKQGH VRSLSERLMQ MSLATSGVKA HPPVTSAPLS

    PPQPNDLYKN PTSSSEFYKA QGPLPNQHSL KGMEHRGPPP EYPFKGMPPQ

    SVVCKPQEPG HFYSEHRLNQ PGRTEGQLMR YQHPPEYGAA RPAQDISLPL

    SARNSQPHSP TSSLTSGGSL PLLQSPPSTR LSPARHPLVP NQGDHSAHLP

    RPQQHFLPNQ AHQGDHYRLS QPGLSQQQQQ QQQQHHHHHH HQQQQQQQPQ

    QQPGEAYSAM PRAQPSSASY QPVPADPFAI VSRAQQMVEI LSDENRNLRQ

    ELEGCYEKVA RLQKVETEIQ RVSEAYENLV KSSSKREALE KAMRNKLEGE

    IRRMHDFNRD LRERLETANK QLAEKEYEGS EDTRKTISQL FAKNKESQRE

    KEKLEAELAT ARSTNEDQRR HIEIRDQALS NAQAKVVKLE EELKKKQVYV

    DKVEKMQQAL VQLQAACEKR EQLEHRLRTR LERELESLRI QQRQGNCQPT

    NVSEYNAAAL MELLREKEER ILALEADMTK WEQKYLEENV MRHFALDAAA

    TVAAQRDTTV ISHSPNTSYD TALEARIQKE EEEILMANKR CLDMEGRIKT

    LHAQIIEKDA MIKVLQQRSR KEPSKTEQLS CMRPAKSLMS ISNAGSGLLS

    HSSTLTGSPI MEEKRDDKSW KGSLGILLGG DYRAEYVPST PSPVPPSTPL

    LSAHSKTGSR DCSTQTERGT ESNKTAAVAP ISVPAPVAAA ATAAAITATA

    ATITTTMVAA APVAVAAAAA PAAAAAPSPA TAAATAAAVS PAAAGQIPAA

    ASVASAAAVA PSAAAAAAVQ VAPAAPAPVP APALVPVPAP AAAQASAPAQ

    TQAPTSAPAV APTPAPTPTP AVAQAEVPAS PATGPGPHRL SIPSLTCNPD

    KTDGPVFHSN TLERKTPIQI LGQEPDAEMV EYLI

  • UCSC uc004epr.4; human. [Q4VCS5-1];


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