Human AMOT ELISA Kit

Alternative name
Human KIAA1071 ELISA Kit;Human angiomotin ELISA Kit;Human angiomotin p130 isoform ELISA Kit;Human angiomotin p80 isoform ELISA Kit;
Catalog
E002026
species
Human
GeneAMOT
Standard Curve
Other Species Mouse Amot ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AMOT. No significant cross-reactivity or interference between AMOT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AMOT and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman AMOT ELISA Kit allows for the in vitro quantitative determination of AMOT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: AMOT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMOT antibody and an AMOT-HRP conjugate. The assay sample and buffer are incubated together with AMOT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMOT concentration since AMOT from samples and AMOT-HRP conjugate compete for the anti-AMOT antibody binding site. Since the number of sites is limited, as more sites are occupied by AMOT from the sample, fewer sites are left to bind AMOT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMOT concentration in each sample is interpolated from this standard curve.
Human Angiomotin Protein information
Uniprot ID AMOT_HUMAN
Uniprot AC Q4VCS5; Q504X5; Q9HD27; Q9UPT1;
UniGene Hs.528051;
GeneID 154796
KEGG hsa:154796;
Human Angiomotin Protein SEQUENCE
SEQUENCE 1084 AA; 118085 MW; D7E7021E9535A628 CRC64;
MRNSEEQPSG GTTVLQRLLQ EQLRYGNPSE NRSLLAIHQQ ATGNGPPFPS
GSGNPGPQSD VLSPQDHHQQ LVAHAARQEP QGQEIQSENL IMEKQLSPRM
QNNEELPTYE EAKVQSQYFR GQQHASVGAA FYVTGVTNQK MRTEGRPSVQ
RLNPGKMHQD EGLRDLKQGH VRSLSERLMQ MSLATSGVKA HPPVTSAPLS
PPQPNDLYKN PTSSSEFYKA QGPLPNQHSL KGMEHRGPPP EYPFKGMPPQ
SVVCKPQEPG HFYSEHRLNQ PGRTEGQLMR YQHPPEYGAA RPAQDISLPL
SARNSQPHSP TSSLTSGGSL PLLQSPPSTR LSPARHPLVP NQGDHSAHLP
RPQQHFLPNQ AHQGDHYRLS QPGLSQQQQQ QQQQHHHHHH HQQQQQQQPQ
QQPGEAYSAM PRAQPSSASY QPVPADPFAI VSRAQQMVEI LSDENRNLRQ
ELEGCYEKVA RLQKVETEIQ RVSEAYENLV KSSSKREALE KAMRNKLEGE
IRRMHDFNRD LRERLETANK QLAEKEYEGS EDTRKTISQL FAKNKESQRE
KEKLEAELAT ARSTNEDQRR HIEIRDQALS NAQAKVVKLE EELKKKQVYV
DKVEKMQQAL VQLQAACEKR EQLEHRLRTR LERELESLRI QQRQGNCQPT
NVSEYNAAAL MELLREKEER ILALEADMTK WEQKYLEENV MRHFALDAAA
TVAAQRDTTV ISHSPNTSYD TALEARIQKE EEEILMANKR CLDMEGRIKT
LHAQIIEKDA MIKVLQQRSR KEPSKTEQLS CMRPAKSLMS ISNAGSGLLS
HSSTLTGSPI MEEKRDDKSW KGSLGILLGG DYRAEYVPST PSPVPPSTPL
LSAHSKTGSR DCSTQTERGT ESNKTAAVAP ISVPAPVAAA ATAAAITATA
ATITTTMVAA APVAVAAAAA PAAAAAPSPA TAAATAAAVS PAAAGQIPAA
ASVASAAAVA PSAAAAAAVQ VAPAAPAPVP APALVPVPAP AAAQASAPAQ
TQAPTSAPAV APTPAPTPTP AVAQAEVPAS PATGPGPHRL SIPSLTCNPD
KTDGPVFHSN TLERKTPIQI LGQEPDAEMV EYLI
UCSC uc004epr.4; human. [Q4VCS5-1];

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Category

Human AMOT ELISA Kit

Human KIAA1071 ELISA Kit;Human angiomotin ELISA Kit;Human angiomotin p130 isoform ELISA Kit;Human angiomotin p80 isoform ELISA Kit;