Human KLF14 ELISA Kit

Alternative name
Human Basic transcription element-binding protein 5 ELISA Kit;Human BTE-binding protein 5 ELISA Kit;Human Transcription factor BTEB5 ELISA Kit;Human BTEB5 ELISA Kit;
Catalog
E021944
species
Human
GeneKLF14
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094nmol/L
Intended UseHuman KLF14 ELISA Kit allows for the in vitro quantitative determination of KLF14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human KLF14 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human KLF14 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLF14 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human KLF14can be calculated.
Human Krueppel-like factor 14 Protein information
Uniprot ID KLF14_HUMAN
Uniprot AC Q8TD94; Q19A42; Q19A43;
UniGene Hs.125240;
Human Krueppel-like factor 14 Protein SEQUENCE
SEQUENCE 323 AA; 33124 MW; D9129E7968E598A3 CRC64;
MSAAVACLDY FAAECLVSMS AGAVVHRRPP DPEGAGGAAG SEVGAAHPES
ALPGPGPSGP ASVPQLPQVP APSPGAGGAA PHLLAASVWA DLRGSSGEGS
WENSGEAPRA SSGFSDPIPC SVQTPCSELA PASGAAAVCA PESSSDAPAV
PSAPAAPGAP AASGGFSGGA LGAGPAPAAD QAPRRRSVTP AAKRHQCPFP
GCTKAYYKSS HLKSHQRTHT GERPFSCDWL DCDKKFTRSD ELARHYRTHT
GEKRFSCPLC PKQFSRSDHL TKHARRHPTY HPDMIEYRGR RRTPRIDPPL
TSEVESSASG SGPGPAPSFT TCL

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Human KLF14 ELISA Kit

Human Basic transcription element-binding protein 5 ELISA Kit;Human BTE-binding protein 5 ELISA Kit;Human Transcription factor BTEB5 ELISA Kit;Human BTEB5 ELISA Kit;