Human IGHG1 ELISA Kit

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  • Catalog
    E019614
  • species
    Human
  • GeneIGHG1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human IGHG1. No significant cross-reactivity or interference between human IGHG1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human IGHG1 is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman IGHG1 ELISA Kit allows for the in vitro quantitative determination of IGHG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGHG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGHG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGHG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGHG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Ig gamma-1 chain C region Protein information
  • Uniprot ID IGHG1_HUMAN
  • Uniprot AC P01857;
  • UniGene Hs.510635;
  • Human Ig gamma-1 chain C region Protein SEQUENCE
  • SEQUENCE 330 AA; 36106 MW; 3770EE106C2FA33D CRC64;

    ASTKGPSVFP LAPSSKSTSG GTAALGCLVK DYFPEPVTVS WNSGALTSGV

    HTFPAVLQSS GLYSLSSVVT VPSSSLGTQT YICNVNHKPS NTKVDKKVEP

    KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS

    HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK

    EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC

    LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW

    QQGNVFSCSV MHEALHNHYT QKSLSLSPGK

  • UCSC uc059gdc.1; human.;



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