Intended UseHuman NGB ELISA Kit allows for the in vitro quantitative determination of NGB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntroduction: Neuroglobin is a member of the vertebrate globin family involved in cellular oxygen homeostasis. It is an intracellular hemoprotein expressed in the central and peripheral nervous system, cerebrospinal fluid, retina and endocrine tissues. Neuroglobin is a monomer that reversibly binds oxygen with an affinity higher than that of hemoglobin. It also increases oxygen availability to brain tissue and provides protection under hypoxic or ischemic conditions, potentially limiting brain damage. It is of ancient evolutionary origin, and is homologous to nerve globins of invertebrates. Neuroglobin was first identified by Thorsten Burmester et al. in 2000. Italian researchers suggest that neuroglobin is more likely to usher in nitric oxide to protect neuron survival and recovery in areas where oxygen supply is reduced. The 3D structure of human neuroglobin was determined in 2003. The next year, murine neuroglobin was determined at a higher resolution.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NGB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NGB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NGB in the samples is then determined by comparing the O.D. of the samples to the standard curve.