Human OPRM1 ELISA Kit

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  • Alternative name

    Human M-OR-1 ELISA Kit;Human MOR-1 ELISA Kit;Human mu opiate receptor ELISA Kit;Human Mu opioid receptor ELISA Kit;Human MOP ELISA Kit;Human hMOP ELISA Kit;Human MOR1 ELISA Kit;Human LMOR ELISA Kit;Human MOR ELISA Kit;Human OPRM ELISA Kit;Human opioid receptor mu 1 ELISA Kit;Human mu-type opioid receptor ELISA Kit;Human mu opioid receptor hMOR-1a ELISA Kit;

  • Catalog
    E025001
  • species
    Human
  • GeneOPRM1
  • Standard CurveHuman OPRM1 ELISA Kit
  • Other Species Mouse Oprm1 ELISA KitBovine OPRM1 ELISA KitGuinea Pig OPRM1 ELISA KitMonkey OPRM1 ELISA KitPorcine OPRM1 ELISA KitRat Oprm1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of OPRM-1. No significant cross-reactivity or interference between OPRM-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OPRM-1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman OPRM1 ELISA Kit allows for the in vitro quantitative determination of OPRM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeuroscience
  • Product Description
    specifical
    Principle of the assay: OPRM-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OPRM-1 antibody and an OPRM-1-HRP conjugate. The assay sample and buffer are incubated together with OPRM-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPRM-1 concentration since OPRM-1 from samples and OPRM-1-HRP conjugate compete for the anti-OPRM-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OPRM-1 from the sample, fewer sites are left to bind OPRM-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPRM-1 concentration in each sample is interpolated from this standard curve.
  • Human Mu-type opioid receptor Protein information
  • Uniprot ID OPRM_HUMAN
  • Uniprot AC Q9UN57;
  • UniGene Hs.2353;
  • GeneID 4988
  • KEGG hsa:4988;
  • Human Mu-type opioid receptor Protein SEQUENCE
  • SEQUENCE 400 AA; 44779 MW; 1DABEBEC9AFF6F66 CRC64;

    MDSSAAPTNA SNCTDALAYS SCSPAPSPGS WVNLSHLDGN LSDPCGPNRT

    DLGGRDSLCP PTGSPSMITA ITIMALYSIV CVVGLFGNFL VMYVIVRYTK

    MKTATNIYIF NLALADALAT STLPFQSVNY LMGTWPFGTI LCKIVISIDY

    YNMFTSIFTL CTMSVDRYIA VCHPVKALDF RTPRNAKIIN VCNWILSSAI

    GLPVMFMATT KYRQGSIDCT LTFSHPTWYW ENLLKICVFI FAFIMPVLII

    TVCYGLMILR LKSVRMLSGS KEKDRNLRRI TRMVLVVVAV FIVCWTPIHI

    YVIIKALVTI PETTFQTVSW HFCIALGYTN SCLNPVLYAF LDENFKRCFR

    EFCIPTSSNI EQQNSTRIRQ NTRDHPSTAN TVDRTNHQLE NLEAETAPLP

  • UCSC uc003qpn.4; human. [P35372-1];



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