Human NKRF ELISA Kit

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  • Alternative name

    Human NFkB-repressing factor ELISA Kit;Human Protein ITBA4 ELISA Kit;Human transcription factor NRF ELISA Kit;Human ITBA4 ELISA Kit;Human NRF ELISA Kit;Human NFKB repressing factor ELISA Kit;Human NF-kappa-B-repressing factor ELISA Kit;

  • Catalog
    E026480
  • species
    Human
  • GeneNKRF
  • Other Species Mouse Nkrf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NKRF. No significant cross-reactivity or interference between human NKRF and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NKRF is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman NKRF ELISA Kit allows for the in vitro quantitative determination of NKRF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NKRF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NKRF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NKRF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NKRF bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human NF-kappa-B-repressing factor Protein information
  • Uniprot ID NKRF_HUMAN
  • Uniprot AC EVRCQNIY LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI
  • UniGene Hs.437084;
  • GeneID 55922
  • KEGG hsa:55922;
  • Human NF-kappa-B-repressing factor Protein SEQUENCE
  • SEQUENCE 690 AA; 77673 MW; 3067A52671A7AE8F CRC64;

    MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK

    FHARPRFEPV HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY

    TQDSFSIQDG NSQYCDSSGF ILTKDQPVTA NMYFDSGNPA PSTTSQQANS

    QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT IWKNLSNPEM TSGSDKINYT

    YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY ACEVRCQNIY

    LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI

    GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI

    GILNNSASFN KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA

    AADEALKILQ KTQPTYPSVK SSQCHTGSSP RGSGKKKDIK DLVVYENSSN

    PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC KVILESEVIA EAVGVKKTVK

    YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS AEEAYKQQIK

    EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK

    IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH

    GVGHDRYLVV GRKRRKEDLL DQLKQEGQVG HYELVMPQAN

  • UCSC uc004erq.3; human. [O15226-1];


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