SpecificityThis assay has high sensitivity and excellent specificity for detection of human ANT autoantibody. No significant cross-reactivity or interference between ANT autoantibody and analogues was observed.
Intended UseHuman ANT ELISA Kit allows for the in vitro quantitative determination of ANT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human ANT autoantibody bound in the initial step. The color development is stopped and the intensity of the color is measured.