Human IL18BP ELISA Kit

Alternative name
Human IL-18BP ELISA Kit;Human tadekinig-alfa ELISA Kit;Human IL18BPa ELISA Kit;Human interleukin 18 binding protein ELISA Kit;Human interleukin-18-binding protein ELISA Kit;Human MC51L-53L-54L homolog gene product ELISA Kit;
Catalog
E019396
species
Human
GeneIL18BP
SpecificityNatural and recombinant human IL-18BP
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman IL18BP ELISA Kit allows for the in vitro quantitative determination of IL18BP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-18BP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18BP has been precoated onto 96-well plates. Standards(NSO, T31-G194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18BP amount of sample captured in plate. Background: Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.
Human Interleukin-18-binding protein Protein information
Uniprot ID I18BP_HUMAN
Uniprot AC O95998; B3KUZ0; B7WPK4; O95993; O96027; Q9NZA9; Q9UBR7;
UniGene Hs.591967;
GeneID 10068
KEGG hsa:10068;
Human Interleukin-18-binding protein Protein SEQUENCE
SEQUENCE 194 AA; 21099 MW; 5E520D6E46AFA843 CRC64;
MTMRHNWTPD LSPLWVLLLC AHVVTLLVRA TPVSQTTTAA TASVRSTKDP
CPSQPPVFPA AKQCPALEVT WPEVEVPLNG TLSLSCVACS RFPNFSILYW
LGNGSFIEHL PGRLWEGSTS RERGSTGTQL CKALVLEQLT PALHSTNFSC
VLVDPEQVVQ RHVVLAQLWA GLRATLPPTQ EALPSSHSSP QQQG
UCSC uc001ore.2; human. [O95998-2];

Category

Human IL18BP ELISA Kit

Human IL-18BP ELISA Kit;Human tadekinig-alfa ELISA Kit;Human IL18BPa ELISA Kit;Human interleukin 18 binding protein ELISA Kit;Human interleukin-18-binding protein ELISA Kit;Human MC51L-53L-54L homolog gene product ELISA Kit;