Human GSS ELISA Kit

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  • Alternative name

    Human GSH synthetase ELISA Kit;Human GSH-S ELISA Kit;Human glutathione synthase ELISA Kit;Human GSHS ELISA Kit;Human HEL-S-64p ELISA Kit;Human HEL-S-88n ELISA Kit;Human glutathione synthetase ELISA Kit;

  • Catalog
    E016961
  • species
    Human
  • GeneGSS
  • Standard CurveHuman GSS ELISA Kit
  • Other Species Mouse Gss ELISA KitBovine GSS ELISA KitMonkey GSS ELISA KitRat Gss ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GSS. No significant cross-reactivity or interference between GSS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human GSS is typically less than 0.127ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
  • Intended UseHuman GSS ELISA Kit allows for the in vitro quantitative determination of GSS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GSS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GSS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Glutathione synthetase Protein information
  • Uniprot ID GSHB_HUMAN
  • Uniprot AC P48637; B2R697; B6F210; E1P5P9; Q4TTD9;
  • UniGene Hs.82327;
  • GeneID 2937
  • KEGG hsa:2937;
  • Human Glutathione synthetase Protein SEQUENCE
  • SEQUENCE 474 AA; 52385 MW; 3C25EF7072EFE058 CRC64;

    MATNWGSLLQ DKQQLEELAR QAVDRALAEG VLLRTSQEPT SSEVVSYAPF

    TLFPSLVPSA LLEQAYAVQM DFNLLVDAVS QNAAFLEQTL SSTIKQDDFT

    ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QRSADGSPAL KQIEINTISA

    SFGGLASRTP AVHRHVLSVL SKTKEAGKIL SNNPSKGLAL GIAKAWELYG

    SPNALVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRTFE DISEKGSLDQ

    DRRLFVDGQE IAVVYFRDGY MPRQYSLQNW EARLLLERSH AAKCPDIATQ

    LAGTKKVQQE LSRPGMLEML LPGQPEAVAR LRATFAGLYS LDVGEEGDQA

    IAEALAAPSR FVLKPQREGG GNNLYGEEMV QALKQLKDSE ERASYILMEK

    IEPEPFENCL LRPGSPARVV QCISELGIFG VYVRQEKTLV MNKHVGHLLR

    TKAIEHADGG VAAGVAVLDN PYPV

  • UCSC uc010zuo.3; human. [P48637-1];



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