Human BIN2 ELISA Kit

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  • Alternative name

    Human breast cancer-associated protein 1 ELISA Kit;Human BRAP1 ELISA Kit;Human BRAP-1 ELISA Kit;Human bridging integrator 2 ELISA Kit;Human breast cancer associated protein BRAP1 ELISA Kit;

  • Catalog
    E005319
  • species
    Human
  • GeneBIN2
  • Standard CurveHuman BIN2 ELISA Kit
  • Other Species Mouse Bin2 ELISA KitChicken BIN2 ELISA KitRat Bin2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Bridging Integrator 2 (BIN2). No significant cross-reactivity or interference between Bridging Integrator 2 (BIN2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.112ng/mL.
  • Intended UseHuman BIN2 ELISA Kit allows for the in vitro quantitative determination of BIN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bridging Integrator 2 (BIN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bridging Integrator 2 (BIN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bridging Integrator 2 (BIN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bridging Integrator 2 (BIN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Bridging integrator 2 Protein information
  • Uniprot ID BIN2_HUMAN
  • Uniprot AC Q9UBW5; Q86VV0; Q9NWK4; Q9UKN4;
  • UniGene Hs.14770;
  • GeneID 51411
  • KEGG hsa:51411;
  • Human Bridging integrator 2 Protein SEQUENCE
  • SEQUENCE 565 AA; 61874 MW; B1D1FB89A97E9E43 CRC64;

    MAEGKAGGAA GLFAKQVQKK FSRAQEKVLQ KLGKAVETKD ERFEQSASNF

    YQQQAEGHKL YKDLKNFLSA VKVMHESSKR VSETLQEIYS SEWDGHEELK

    AIVWNNDLLW EDYEEKLADQ AVRTMEIYVA QFSEIKERIA KRGRKLVDYD

    SARHHLEAVQ NAKKKDEAKT AKAEEEFNKA QTVFEDLNQE LLEELPILYN

    SRIGCYVTIF QNISNLRDVF YREMSKLNHN LYEVMSKLEK QHSNKVFVVK

    GLSSSSRRSL VISPPVRTAT VSSPLTSPTS PSTLSLKSES ESVSATEDLA

    PDAAQGEDNS EIKELLEEEE IEKEGSEASS SEEDEPLPAC NGPAQAQPSP

    TTERAKSQEE VLPSSTTPSP GGALSPSGQP SSSATEVVLR TRTASEGSEQ

    PKKRASIQRT SAPPSRPPPP RATASPRPSS GNIPSSPTAS GGGSPTSPRA

    SLGTGTASPR TSLEVSPNPE PPEKPVRTPE AKENENIHNQ NPEELCTSPT

    LMTSQVASEP GEAKKMEDKE KDNKLISANS SEGQDQLQVS MVPENNNLTA

    PEPQEEVSTS ENPQL

  • UCSC uc058ocp.1; human. [Q9UBW5-1];


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