Human HGD ELISA Kit

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  • Alternative name

    Human homogentisate oxygenase ELISA Kit;Human homogentisic acid oxidase ELISA Kit;Human homogentisicase ELISA Kit;Human HGO ELISA Kit;Human AKU ELISA Kit;Human homogentisate 1,2-dioxygenase ELISA Kit;Human homogentisate oxidase ELISA Kit;

  • Catalog
    E018949
  • species
    Human
  • GeneHGD
  • Standard CurveHuman HGD ELISA Kit
  • Other Species Mouse Hgd ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Homogentisate-1,2-Dioxygenase (HGD). No significant cross-reactivity or interference between Homogentisate-1,2-Dioxygenase (HGD) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman HGD ELISA Kit allows for the in vitro quantitative determination of HGD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homogentisate-1,2-Dioxygenase (HGD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Homogentisate-1,2-Dioxygenase (HGD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Homogentisate-1,2-Dioxygenase (HGD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Homogentisate-1,2-Dioxygenase (HGD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Homogentisate 1,2-dioxygenase Protein information
  • Uniprot ID HGD_HUMAN
  • Uniprot AC Q93099; A8K417; B2R8Z0;
  • UniGene Hs.368254;
  • GeneID 3081
  • KEGG hsa:3081;
  • Human Homogentisate 1,2-dioxygenase Protein SEQUENCE
  • SEQUENCE 445 AA; 49964 MW; F99B51C134FFF965 CRC64;

    MAELKYISGF GNECSSEDPR CPGSLPEGQN NPQVCPYNLY AEQLSGSAFT

    CPRSTNKRSW LYRILPSVSH KPFESIDEGQ VTHNWDEVDP DPNQLRWKPF

    EIPKASQKKV DFVSGLHTLC GAGDIKSNNG LAIHIFLCNT SMENRCFYNS

    DGDFLIVPQK GNLLIYTEFG KMLVQPNEIC VIQRGMRFSI DVFEETRGYI

    LEVYGVHFEL PDLGPIGANG LANPRDFLIP IAWYEDRQVP GGYTVINKYQ

    GKLFAAKQDV SPFNVVAWHG NYTPYKYNLK NFMVINSVAF DHADPSIFTV

    LTAKSVRPGV AIADFVIFPP RWGVADKTFR PPYYHRNCMS EFMGLIRGHY

    EAKQGGFLPG GGSLHSTMTP HGPDADCFEK ASKVKLAPER IADGTMAFMF

    ESSLSLAVTK WGLKASRCLD ENYHKCWEPL KSHFTPNSRN PAEPN

  • UCSC uc003edw.4; human.;


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