Human HGD ELISA Kit

Alternative name
Human homogentisate oxygenase ELISA Kit;Human homogentisic acid oxidase ELISA Kit;Human homogentisicase ELISA Kit;Human HGO ELISA Kit;Human AKU ELISA Kit;Human homogentisate 1,2-dioxygenase ELISA Kit;Human homogentisate oxidase ELISA Kit;
Catalog
E018949
species
Human
GeneHGD
SpecificityThis assay has high sensitivity and excellent specificity for detection of Homogentisate-1,2-Dioxygenase (HGD). No significant cross-reactivity or interference between Homogentisate-1,2-Dioxygenase (HGD) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.063ng/mL.
Intended UseHuman HGD ELISA Kit allows for the in vitro quantitative determination of HGD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homogentisate-1,2-Dioxygenase (HGD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Homogentisate-1,2-Dioxygenase (HGD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Homogentisate-1,2-Dioxygenase (HGD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Homogentisate-1,2-Dioxygenase (HGD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Homogentisate 1,2-dioxygenase Protein information
Uniprot ID HGD_HUMAN
Uniprot AC Q93099; A8K417; B2R8Z0;
UniGene Hs.368254;
GeneID 3081
KEGG hsa:3081;
Human Homogentisate 1,2-dioxygenase Protein SEQUENCE
SEQUENCE 445 AA; 49964 MW; F99B51C134FFF965 CRC64;
MAELKYISGF GNECSSEDPR CPGSLPEGQN NPQVCPYNLY AEQLSGSAFT
CPRSTNKRSW LYRILPSVSH KPFESIDEGQ VTHNWDEVDP DPNQLRWKPF
EIPKASQKKV DFVSGLHTLC GAGDIKSNNG LAIHIFLCNT SMENRCFYNS
DGDFLIVPQK GNLLIYTEFG KMLVQPNEIC VIQRGMRFSI DVFEETRGYI
LEVYGVHFEL PDLGPIGANG LANPRDFLIP IAWYEDRQVP GGYTVINKYQ
GKLFAAKQDV SPFNVVAWHG NYTPYKYNLK NFMVINSVAF DHADPSIFTV
LTAKSVRPGV AIADFVIFPP RWGVADKTFR PPYYHRNCMS EFMGLIRGHY
EAKQGGFLPG GGSLHSTMTP HGPDADCFEK ASKVKLAPER IADGTMAFMF
ESSLSLAVTK WGLKASRCLD ENYHKCWEPL KSHFTPNSRN PAEPN
UCSC uc003edw.4; human.;

Category

Human HGD ELISA Kit

Human homogentisate oxygenase ELISA Kit;Human homogentisic acid oxidase ELISA Kit;Human homogentisicase ELISA Kit;Human HGO ELISA Kit;Human AKU ELISA Kit;Human homogentisate 1,2-dioxygenase ELISA Kit;Human homogentisate oxidase ELISA Kit;